3bjz: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="3bjz" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bjz, resolution 2.40Å" /> '''Crystal structure of... |
No edit summary |
||
| Line 2: | Line 2: | ||
caption="3bjz, resolution 2.40Å" /> | caption="3bjz, resolution 2.40Å" /> | ||
'''Crystal structure of Pseudomonas aeruginosa phosphoheptose isomerase'''<br /> | '''Crystal structure of Pseudomonas aeruginosa phosphoheptose isomerase'''<br /> | ||
==Overview== | |||
The barrier imposed by lipopolysaccharide (LPS) in the outer membrane of, Gram-negative bacteria presents a significant challenge in treatment of, these organisms with otherwise effective hydrophobic antibiotics. The, absence of l-glycero-d-manno-heptose in the LPS molecule is associated, with a dramatically increased bacterial susceptibility to hydrophobic, antibiotics and thus enzymes in the ADP-heptose biosynthesis pathway are, of significant interest. GmhA catalyzes the isomerization of, d-sedoheptulose 7-phosphate into d-glycero-d-manno-heptose 7-phosphate, the first committed step in the formation of ADP-heptose. Here we report, structures of GmhA from Escherichia coli and Pseudomonas aeruginosa in, apo, substrate, and product-bound forms, which together suggest that GmhA, adopts two distinct conformations during isomerization through, reorganization of quaternary structure. Biochemical characterization of, GmhA mutants, combined with in vivo analysis of LPS biosynthesis and, novobiocin susceptibility, identifies key catalytic residues. We postulate, GmhA acts through an enediol-intermediate isomerase mechanism. | |||
==About this Structure== | ==About this Structure== | ||
3BJZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJZ OCA]. | 3BJZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:So4 Binding Site For Residue A 198'>AC1</scene>, <scene name='pdbsite=AC2:So4 Binding Site For Residue B 198'>AC2</scene>, <scene name='pdbsite=AC3:So4 Binding Site For Residue C 198'>AC3</scene> and <scene name='pdbsite=AC4:So4 Binding Site For Residue D 198'>AC4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJZ OCA]. | ||
==Reference== | |||
Structure and Function of Sedoheptulose-7-phosphate Isomerase, a Critical Enzyme for Lipopolysaccharide Biosynthesis and a Target for Antibiotic Adjuvants., Taylor PL, Blakely KM, de Leon GP, Walker JR, McArthur F, Evdokimova E, Zhang K, Valvano MA, Wright GD, Junop MS, J Biol Chem. 2008 Feb 1;283(5):2835-45. Epub 2007 Dec 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18056714 18056714] | |||
[[Category: Pseudomonas aeruginosa pao1]] | [[Category: Pseudomonas aeruginosa pao1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 27: | Line 33: | ||
[[Category: sis domain]] | [[Category: sis domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 31 11:02:38 2008'' | ||
