Sandbox 31: Difference between revisions

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The active site of papain has a <scene name='Sandbox_31/Activesite/1'>"Catalytic Triad"</scene>including CYS 25, HIS 159, and ASN 175.  
The active site of papain has a <scene name='Sandbox_31/Activesite/1'>"Catalytic Triad"</scene>including CYS 25, HIS 159, and ASN 175.  
[[Image:papain2.jpg]]  
[[Image:papain2.jpg]]  
  This triad interacts with the substrate to catalyze the reaction.  The sulfur from CYS 25 attacks the backbone amine on the substrate forming a tetrahedral intermediate.  Next, the carbonyl is reformed and the carbon nitrogen bond is broken.  A water associated with a nitrogen on HIS 159 then attacks the carbonyl forming a second tetrahedral intermediate.  The carbonyl then reforms  breaking the carbon-sulfur bond.  This leaves a carboxy group on the end of one piece of the substrate and an amino group on the end of the other piece.
   
 


==Inhibitors==
==Inhibitors==
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