Sandbox 32: Difference between revisions
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Papain is inactivated by oxygen iodine, hydrogen peroxide, and EDTA (4). Also, it is is irreversibly inhibited by N-ethylmaleimide and iodoacetate. Other inhibitors of Papain are <scene name='Sandbox_32/Inhibition/1'>ICP</scene> (inhibitor of cysteine protease) and <scene name='Sandbox_32/Khq/1'>ZLFG-DAM </scene> (1). With ZLFG-DAM(diazomethylketone), the methylene carbon will covalently bind to the cysteine-25 carbon and the hydrophobic pocket near the active site will also be inhibited (1). | Papain is inactivated by oxygen iodine, hydrogen peroxide, and EDTA (4). Also, it is is irreversibly inhibited by N-ethylmaleimide and iodoacetate. Other inhibitors of Papain are <scene name='Sandbox_32/Inhibition/1'>ICP</scene> (inhibitor of cysteine protease) and <scene name='Sandbox_32/Khq/1'>ZLFG-DAM </scene> (1). With ZLFG-DAM(diazomethylketone), the methylene carbon will covalently bind to the cysteine-25 carbon and the hydrophobic pocket near the active site will also be inhibited (1). | ||
This scene should show the contacts between papain and its inhibitor <scene name='Sandbox_32/Inhibitor/1'>ZLFG-DAM</scene> | This scene should show the contacts between papain and its inhibitor <scene name='Sandbox_32/Inhibitor/1'>ZLFG-DAM</scene> | ||
Water and methanol can be considered other ligands to Papain. When obtaining the crystal structure of the enzyme, the solvent becomes part of the stabilized structure. Another ligand that can be considered for Papain is water. Water molecules play an important role in maintaining structural stability. There are 21 water molecules that are located in contact areas between adjacent papain molecule (2). | |||
=='''References'''== | =='''References'''== | ||
