1w5b: Difference between revisions
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[[Image:1w5b.jpg|left|200px]]<br /><applet load="1w5b" size=" | [[Image:1w5b.jpg|left|200px]]<br /><applet load="1w5b" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1w5b, resolution 2.2Å" /> | caption="1w5b, resolution 2.2Å" /> | ||
'''FTSZ DIMER, GTP SOAK (M. JANNASCHII)'''<br /> | '''FTSZ DIMER, GTP SOAK (M. JANNASCHII)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1W5B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with GTP as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Gtp Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | 1W5B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=GTP:'>GTP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Gtp+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5B OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: z-ring]] | [[Category: z-ring]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:19:31 2008'' | ||
Revision as of 08:19, 3 February 2008
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FTSZ DIMER, GTP SOAK (M. JANNASCHII)
Overview
The prokaryotic tubulin homolog FtsZ polymerizes into a ring structure, essential for bacterial cell division. We have used refolded FtsZ to, crystallize a tubulin-like protofilament. The N- and C-terminal domains of, two consecutive subunits in the filament assemble to form the GTPase site, with the C-terminal domain providing water-polarizing residues. A, domain-swapped structure of FtsZ and biochemical data on purified N- and, C-terminal domains show that they are independent. This leads to a model, of how FtsZ and tubulin polymerization evolved by fusing two domains. In, polymerized tubulin, the nucleotide-binding pocket is occluded, which, leads to nucleotide exchange being the rate-limiting step and to dynamic, instability. In our FtsZ filament structure the nucleotide is, exchangeable, explaining why, in this filament, nucleotide hydrolysis is, the rate-limiting step during FtsZ polymerization. Furthermore, crystal, structures of FtsZ in different nucleotide states reveal notably few, differences.
About this Structure
1W5B is a Single protein structure of sequence from Methanocaldococcus jannaschii with GTP as ligand. Known structural/functional Site: AC1. Full crystallographic information is available from OCA.
Reference
Structural insights into FtsZ protofilament formation., Oliva MA, Cordell SC, Lowe J, Nat Struct Mol Biol. 2004 Dec;11(12):1243-50. Epub 2004 Nov 21. PMID:15558053
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