1wae: Difference between revisions
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[[Image:1wae.gif|left|200px]]<br /><applet load="1wae" size=" | [[Image:1wae.gif|left|200px]]<br /><applet load="1wae" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1wae, resolution 1.95Å" /> | caption="1wae, resolution 1.95Å" /> | ||
'''CRYSTAL STRUCTURE OF H129V MUTANT OF ALCALIGENES XYLOSOXIDANS NITRITE REDUCTASE'''<br /> | '''CRYSTAL STRUCTURE OF H129V MUTANT OF ALCALIGENES XYLOSOXIDANS NITRITE REDUCTASE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1WAE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Achromobacter_xylosoxidans Achromobacter xylosoxidans] with CU and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_1.7.2.1 Transferred entry: 1.7.2.1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.3 1.7.99.3] Known structural/functional Site: <scene name='pdbsite=AC1:Zn Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | 1WAE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Achromobacter_xylosoxidans Achromobacter xylosoxidans] with <scene name='pdbligand=CU:'>CU</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_1.7.2.1 Transferred entry: 1.7.2.1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.3 1.7.99.3] Known structural/functional Site: <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAE OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: reductase]] | [[Category: reductase]] | ||
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Revision as of 08:21, 3 February 2008
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CRYSTAL STRUCTURE OF H129V MUTANT OF ALCALIGENES XYLOSOXIDANS NITRITE REDUCTASE
Overview
Copper nitrite reductases contain both an electron-transfer type 1 Cu site, and a catalytic type 2 Cu site. We have mutated one of the type 2 copper, ligating histidines to observe the effect on catalytic turnover. This, mutation has created a unique site where Cu is ligated by 2 His Nepsilon2, atoms alone.
About this Structure
1WAE is a Single protein structure of sequence from Achromobacter xylosoxidans with CU and ZN as ligands. Active as Transferred entry: 1.7.2.1, with EC number 1.7.99.3 Known structural/functional Site: AC1. Full crystallographic information is available from OCA.
Reference
Observation of an unprecedented Cu Bis-His site: crystal structure of the H129V mutant of nitrite reductase., Ellis MJ, Antonyuk SV, Strange RW, Sawers G, Eady RR, Hasnain SS, Inorg Chem. 2004 Nov 29;43(24):7591-3. PMID:15554622
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