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| ==Mechanism of Bio luminescence== | | ==Mechanism of Bio luminescence== |
| Luciferase found in ''''Italic tex<StructureSection load='3fgc' size='500' side='right' caption='Structure of Bacterial Luciferase and FMN complex from ''V. harveyi'' (PDB entry [[3fgc]])' scene=''>
| | Luciferase found in ''''Vibrio harveyi'''' is a heterodimer that is composed of a catalytic α subunit and a homologous but noncatalytic beta subunit. The catalytic |
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| ==Introduction==
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| Luciferases are a class of enzymes that catalyze the oxidation of a long chain aliphatic aldehydes. This reaction results in the formation of a carboxylic acid and the emission of photons in the form of blue-green light.
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| </StructureSection>
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| {{STRUCTURE_3fgc| PDB=3fgc | SCENE= }}
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| ==Mechanism of Bio luminescence==
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| Luciferase found in ''''Vibrio harveyi'''' is a heterodimeric flavin monoxygenase that is composed of a catalytic α subunit and a homologous but noncatalytic β subunit. The catalytic α subunit is bound to a Flavin Mononucleotide cofactor. Bioluminescense is achieved by a two step reaction between | |
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| Active Site | | Active Site |
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| Mobile Loop-The mobile loop of luciferase is thought to be responsible for protecting reaction intermediates and preventing the entry of bulk solvent into the active center once the flavin substrate is bound. | | Mobile Loop |
| | | (β/α)<SUP>8</SUP> Barrel |
| (β/α)&sub8; Barrel- | |
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| Interface between Alpha and Beta Subunits | | Interface between Alpha and Beta Subunits |
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| ==Applications In Biotechnology== | | ==Applications In Biotechnology== |
