User:Mitchell Long/Sandbox 1: Difference between revisions
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==Mechanism of Bio luminescence== | ==Mechanism of Bio luminescence== | ||
Luciferase found in ''''Vibrio harveyi'''' is a heterodimer that is composed of a catalytic α subunit and a homologous but noncatalytic | Luciferase found in ''''Vibrio harveyi'''' is a heterodimer that is composed of a catalytic α subunit and a homologous but noncatalytic β subunit. The catalytic α subunit houses the FMN cofactor and is connected to the β subunit via a hairpin structure called the "mobile loop." | ||
==Structural Motifs== | ==Structural Motifs== | ||
Structure homology-There is a great deal of sequence homology and structural coservation between the α and β subunits. When superimposed over eachother the barrels of the alpha and beta subunits with a deviation of 0.62Å for 42 equivalent | |||
α carbons. | |||
Active Site | Active Site | ||
Mobile Loop | Mobile Loop- Phe2772-thr 288 | ||
(β/α)<SUB>8</SUB> Barrel | (β/α)<SUB>8</SUB> Barrel- The tertiary structure of the α and β subunits is very similar. both subunits fold into a single-domain eight-stranded β/α barrel motif. the two subunits assemble around a parallel four-helix bundle centered on a pseudo 2-fold axis that relates the alpha and beta subunits. | ||
Subunit Homology-the topology of the αand β subunits is identical. In the alpha subnunit, the γ7-α7 loop is 71 residues long and contains 29 residues not present in the beta subunit. | |||
==Applications In Biotechnology== | ==Applications In Biotechnology== | ||
