Ferguson ZNF Sandbox: Difference between revisions

Line 19:

====DNA Binding====

In TFIIIA, there are nine consecutive zinc fingers. Individual zinc fingers can be positioned in both the major groove and across the minor groove of DNA. These results show how TFIIIA can recognize several separated DNA sequences by using fewer fingers than necessary for continuous winding in the major groove. <scene name='Ferguson_ZNF_Sandbox/1tf6/3'>1tf6</scene> shows a dimer with 6 zinc fingers on each bound to a stretch of DNA. With this interaction, TFIIIA helps control the transcription of the gene for ribosomal RNA. The string of zinc fingers curls along the DNA or RNA strands, binding in the grooves and extending amino acids inwards to read the bases. A single zinc finger does not bind very tightly and can only recognize 2 or 3 base pairs, but several can be strung together, causing the group to bind more tightly and allows it to read longer DNA sequences. This modular approach is so appealing that researchers are currently trying to design artificial zinc fingers with different specificities<ref>PMID:2503871</ref>.

In TFIIIA, there are nine consecutive zinc fingers. Individual zinc fingers can be positioned in both the major groove and across the minor groove of DNA. These results show how TFIIIA can recognize several separated DNA sequences by using fewer fingers than necessary for continuous winding in the major groove. <scene name='Ferguson_ZNF_Sandbox/1tf6/3'>1tf6</scene> shows a dimer with 6 zinc fingers on each bound to a stretch of DNA. With this interaction, TFIIIA helps control the transcription of the gene for ribosomal RNA. The string of zinc fingers curls along the DNA or RNA strands, binding in the grooves and <scene name='Zinc_Fingers/Zinc_fingers_1zaa_dna/4'>extending amino acids inwards</scene> to read the bases. A single zinc finger does not bind very tightly and can only recognize 2 or 3 base pairs, but several can be strung together, causing the group to bind more tightly and allows it to read longer DNA sequences. This modular approach is so appealing that researchers are currently trying to design artificial zinc fingers with different specificities<ref>PMID:2503871</ref>.

=====Specificity=====

Ferguson ZNF Sandbox: Difference between revisions

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 19: / Line 19: @@
 ====DNA Binding====
-In TFIIIA, there are nine consecutive zinc fingers.  Individual zinc fingers can be positioned in both the major groove and across the minor groove of DNA. These results show how TFIIIA can recognize several separated DNA sequences by using fewer fingers than necessary for continuous winding in the major groove.  <scene name='Ferguson_ZNF_Sandbox/1tf6/3'>1tf6</scene> shows a dimer with 6 zinc fingers on each bound to a stretch of DNA.  With this interaction, TFIIIA helps control the transcription of the gene for ribosomal RNA.  The string of zinc fingers curls along the DNA or RNA strands, binding in the grooves and extending amino acids inwards to read the bases. A single zinc finger does not bind very tightly and can only recognize 2 or 3 base pairs, but several can be strung together, causing the group to bind more tightly and allows it to read longer DNA sequences. This modular approach is so appealing that researchers are currently trying to design artificial zinc fingers with different specificities<ref>PMID:2503871</ref>.
+In TFIIIA, there are nine consecutive zinc fingers.  Individual zinc fingers can be positioned in both the major groove and across the minor groove of DNA. These results show how TFIIIA can recognize several separated DNA sequences by using fewer fingers than necessary for continuous winding in the major groove.  <scene name='Ferguson_ZNF_Sandbox/1tf6/3'>1tf6</scene> shows a dimer with 6 zinc fingers on each bound to a stretch of DNA.  With this interaction, TFIIIA helps control the transcription of the gene for ribosomal RNA.  The string of zinc fingers curls along the DNA or RNA strands, binding in the grooves and <scene name='Zinc_Fingers/Zinc_fingers_1zaa_dna/4'>extending amino acids inwards</scene> to read the bases. A single zinc finger does not bind very tightly and can only recognize 2 or 3 base pairs, but several can be strung together, causing the group to bind more tightly and allows it to read longer DNA sequences. This modular approach is so appealing that researchers are currently trying to design artificial zinc fingers with different specificities<ref>PMID:2503871</ref>.
 =====Specificity=====