Ferguson ZNF Sandbox: Difference between revisions
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A breakthrough in the studies of zinc finger proteins has been the ability to cleave sites in a large genome through endonuclease activities<ref>MMMMMM</ref>. FokI endonuclease has two domans; one that binds to DNA and another that cleaves the DNA. By fusing a FokI mononmer with two zinc finger proteins, which bind adjacent sequences, will generate at least an 18 base pair sequence specific DNA nuclease that allows for selective targeting in mammalian genomes<ref>Davis, David, and David Stokoe. "Zinc Finger Nucleases as Tools to Understand and Treat Human Diseases." BMC Medicine 8.1 (2010): 42.</ref>. | A breakthrough in the studies of zinc finger proteins has been the ability to cleave sites in a large genome through endonuclease activities<ref>MMMMMM</ref>. FokI endonuclease has two domans; one that binds to DNA and another that cleaves the DNA. By fusing a FokI mononmer with two zinc finger proteins, which bind adjacent sequences, will generate at least an 18 base pair sequence specific DNA nuclease that allows for selective targeting in mammalian genomes<ref>Davis, David, and David Stokoe. "Zinc Finger Nucleases as Tools to Understand and Treat Human Diseases." BMC Medicine 8.1 (2010): 42.</ref>. | ||
[[Image:ZFN DNA Scissors.jpg|center|300px]] | |||
As seen in the figure above, two zinc finger trimers are bound to the DNA sequence creating a dimer of FokI. Each zinc finger binds to nine or more nucleotides. After the cleavage by this complex, overhanging ends are left, which sometimes results in deletions or insertions. If this occurs in a coding region, the outcome could be a shift in the reading frame, which can lead to a null allele of the gene that is targeted<ref></ref>. | As seen in the figure above, two zinc finger trimers are bound to the DNA sequence creating a dimer of FokI. Each zinc finger binds to nine or more nucleotides. After the cleavage by this complex, overhanging ends are left, which sometimes results in deletions or insertions. If this occurs in a coding region, the outcome could be a shift in the reading frame, which can lead to a null allele of the gene that is targeted<ref></ref>. | ||
