Sand box 211: Difference between revisions
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3.↑ Mark R.Tock, Elaine Frary, Jon R.Sayers and Jane A.Grasby. Dynamic evidence for metal ion catalysis in the reaction mediated by a flap endonucleas. The EMBO Journal Vol. 22 No. 5 pp. 995±1004, 200. doi:10.1093/emboj/cdg098[http://www.nature.com/emboj/journal/v22/n5/pdf/7595009a.pdf] | 3.↑ Mark R.Tock, Elaine Frary, Jon R.Sayers and Jane A.Grasby. Dynamic evidence for metal ion catalysis in the reaction mediated by a flap endonucleas. The EMBO Journal Vol. 22 No. 5 pp. 995±1004, 200. doi:10.1093/emboj/cdg098[http://www.nature.com/emboj/journal/v22/n5/pdf/7595009a.pdf] | ||
4.↑ Tock MR, Frary E, Sayers JR, Grasby JA. Dynamic evidence for metal ion catalysis in the reaction mediated by a flap endonuclease EMBO J. 2003 Mar 3;22(5):995-1004. PMID: 12606565 doi: 10.1093/emboj/cdg098 | |||
5.↑ Dervan JJ, Feng M, Patel D, Grasby JA, Artymiuk PJ, Ceska TA, Sayers JR. Interactions of mutant and wild-type flap endonucleases with oligonucleotide substrates suggest an alternative model of DNA binding. Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8542-7 PMID: 12084915 doi: 10.1073/pnas.082241699 | |||
6.↑ Pickering TJ, Garforth S, Sayers JR, Grasby JA. Variation in the steady state kinetic parameters of wild type and mutant T5 5'-3'-exonuclease with pH. Protonation of Lys-83 is critical for DNA binding. J Biol Chem. 1999 Jun 18;274(25):17711-7. PMID: 10364212 doi:10.1074/jbc.274.25.17711 | |||
7.↑ Garforth SJ, Patel D, Feng M, Sayers JR. Unusually wide co-factor tolerance in a metalloenzyme; divalent metal ions modulate endo-exonuclease activity in T5 exonuclease. Nucleic Acids Res. 2001 Jul 1;29(13):2772-9. PMID: 11433022 doi: 10.1093/nar/29.13.2772 | |||
8.↑ Sengerová B, Tomlinson C, Atack JM, Williams R, Sayers JR, Williams NH, Grasby JA. Brønsted analysis and rate-limiting steps for the T5 flap endonuclease catalyzed hydrolysis of exonucleolytic substrates. Biochemistry. 2010 Sep 21;49(37):8085-93. PMID: 20698567 doi: 10.1021/bi100895j | |||
9.↑ Tomlinson CG, Syson K, Sengerová B, Atack JM, Sayers JR, Swanson L, Tainer JA, Williams NH, Grasby JA. Neutralizing mutations of carboxylates that bind metal 2 in T5 flap endonuclease result in an enzyme that still requires two metal ions. J Biol Chem. 2011 Sep 2;286(35):30878-87. Epub 2011 Jul 6. PMID: 21734257 doi: 10.1074/jbc.M111.230391 | |||
10.↑ Pickering TJ, Garforth SJ, Thorpe SJ, Sayers JR, Grasby JA. A single cleavage assay for T5 5'-->3' exonuclease: determination of the catalytic parameters forwild-type and mutant proteins. Nucleic Acids Res. 1999 Feb 1;27(3):730-5. PMID: 9889266 doi: 10.1093/nar/27.3.730 | |||
11.↑ Karl Syson, Christopher Tomlinson, Brian R. Chapados, Jon R. Sayers, John A. Tainer, Nicholas H. Williams and Jane A. Grasby. Three Metal Ions Participate in the Reaction Catalyzed by T5 Flap Endonuclease. J Biol Chem. 2008 October 17; 283(42): 28741–28746. PMCID: PMC2568906 doi: 10.1074/jbc.M801264200 | |||
