Sand box 211: Difference between revisions

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<scene name='Sand_box_211/1exn/4'>T5 5'-exonuclease</scene> is a homodimeric protein composed of two identical chains,<scene name='Sand_box_211/Vghjvjh/4'>chain a</scene> and <scene name='Sand_box_211/Vhj/3'>chain b</scene>.

Both chains contain a hole, bound by a helical arch composed of two helices in which hydrophobic and positively charged residues are located. The helical arch is situated in front of the active site and only single-stranded DNA can pass through it. Since the enzyme is able to cleave double-stranded DNA, the enzyme has a conformational flexibility to facilitate DNA threading which is required to process the 5' nuclease substrates in the active site.

Both chains contain a hole, bound by a <scene name='Celina_Pinto/Sandbox_211/Helical_arch/2'>helical arch</scene> composed of two helices in which hydrophobic and positively charged residues are located. The helical arch is situated in front of the active site and only single-stranded DNA can pass through it. Since the enzyme is able to cleave double-stranded DNA, the enzyme has a conformational flexibility to facilitate DNA threading which is required to process the 5' nuclease substrates in the active site.

The core regions of the enzyme are composed of beta-sheets and alpha-helices . These form the base of the active site pocket which bind the catalytic metal ions.

Sand box 211: Difference between revisions

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@@ Line 21: / Line 21: @@
 <Structure load='1EXN' size='500' frame='true' align='right' caption='T5 5'exonuclease' scene='Insert optional scene name here' />
 <scene name='Sand_box_211/1exn/4'>T5 5'-exonuclease</scene> is a homodimeric protein composed of two identical chains,<scene name='Sand_box_211/Vghjvjh/4'>chain a</scene> and <scene name='Sand_box_211/Vhj/3'>chain b</scene>.
-Both chains contain a hole, bound by a helical arch composed of two helices in which hydrophobic and positively charged residues are located. The helical arch is situated in front of the active site and only single-stranded DNA can pass through it. Since the enzyme is able to cleave double-stranded DNA, the enzyme has a conformational flexibility to facilitate DNA threading which is required to process the 5' nuclease substrates in the active site.
+Both chains contain a hole, bound by a  <scene name='Celina_Pinto/Sandbox_211/Helical_arch/2'>helical arch</scene> composed of two helices in which hydrophobic and positively charged residues are located. The helical arch is situated in front of the active site and only single-stranded DNA can pass through it. Since the enzyme is able to cleave double-stranded DNA, the enzyme has a conformational flexibility to facilitate DNA threading which is required to process the 5' nuclease substrates in the active site.
 The core regions of the enzyme are composed of beta-sheets and alpha-helices . These form the base of the active site pocket which bind the catalytic metal ions.