Sandbox 666: Difference between revisions
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The reaction is due to a catalytic sequence motif which is found in most type II restriction endonucleases: the PD…D/EXK motif. For EcoRI,<scene name='Sandbox_666/Catalytic_core/3'>this catalytic sequence</scene> is PD<sup>91</sup> …E<sup>111</sup>AK. This motif is also responsible for Mg2+ binding(Asp90 and Glu111). [[Image:Catalytic 1ERI.png | thumb | The catalytic core of Eco RI with the target DNA]]<ref> | The reaction is due to a catalytic sequence motif which is found in most type II restriction endonucleases: the PD…D/EXK motif. For EcoRI,<scene name='Sandbox_666/Catalytic_core/3'>this catalytic sequence</scene> is PD<sup>91</sup> …E<sup>111</sup>AK. This motif is also responsible for Mg2+ binding(Asp90 and Glu111). [[Image:Catalytic 1ERI.png | thumb | The catalytic core of Eco RI with the target DNA|left|500px]]<ref> | ||
Structure and function of type II restriction endonucleases | Structure and function of type II restriction endonucleases | ||
Alfred Pingoud, Albert Jeltsch | Alfred Pingoud, Albert Jeltsch | ||
