Sandbox 666: Difference between revisions

Revision as of 13:34, 29 December 2011 view source Virginie Grosboillot (talk \| contribs) 52 edits →Catalytic Mechanism ← Older edit		Revision as of 13:34, 29 December 2011 view source Virginie Grosboillot (talk \| contribs) 52 edits →Application of EcoRI in molecular biology Newer edit →
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	Type II restriction endonuclease like ~~EcoRI~~ are often used in molecular biology for their capacity to cut precisely DNA on specific restriction site. That makes them useful tools for gene cloning. By using two different restriction enzymes, it is possible to do directional cloning, which is very important if you want to insert a gene in an expression vector.		Type II restriction endonuclease like ''Eco''RI are often used in molecular biology for their capacity to cut precisely DNA on specific restriction site. That makes them useful tools for gene cloning. By using two different restriction enzymes, it is possible to do directional cloning, which is very important if you want to insert a gene in an expression vector.

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