Sandbox 666: Difference between revisions
From Proteopedia
Jump to navigationJump to search
| Line 26: | Line 26: | ||
The reaction is due to a catalytic sequence motif which is found in most type II restriction endonucleases: the PD…(D/E)XK motif. For ''Eco''RI,<scene name='Sandbox_666/Catalytic_core/3'>this catalytic sequence</scene> is PD<sup>91</sup> …E<sup>111</sup>AK. This motif is also responsible for Mg2+ binding(Asp90 and Glu111). | The reaction is due to a catalytic sequence motif which is found in most type II restriction endonucleases: the PD…(D/E)XK motif. For ''Eco''RI,<scene name='Sandbox_666/Catalytic_core/3'>this catalytic sequence</scene> is PD<sup>91</sup> …E<sup>111</sup>AK and the lysine residue is essential to the catalysis, but the proline residue is not important. This motif is also responsible for Mg2+ binding(Asp90 and Glu111). | ||
[[Image:Catalytic 1ERI.png | thumb | The catalytic core of Eco RI with the target DNA|left|400px]]<ref> | [[Image:Catalytic 1ERI.png | thumb | The catalytic core of Eco RI with the target DNA|left|400px]]<ref> | ||
Structure and function of type II restriction endonucleases | Structure and function of type II restriction endonucleases | ||
