Sandbox 666: Difference between revisions
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''Eco''RI is a type II restriction endonuclease. It recognizes and cleaves DNA on a specific palindromic sequence: GAATTC. ''Eco''RI has been extracted from strain R ''Escherichia coli'', a common bacterium, which populates the intestine of mammalians. In bacteria, restriction enzymes protect the cell by cutting foreign DNA from bacteriophages (specific bacterial viruses) in both strands. Bacterial DNA is protected by a specific methylation of ''Eco''RI recognition sequences. | ''Eco''RI is a type II restriction endonuclease. It recognizes and cleaves DNA on a specific palindromic sequence: GAATTC. ''Eco''RI has been extracted from strain R ''Escherichia coli'', a common bacterium, which populates the intestine of mammalians. In bacteria, restriction enzymes protect the cell by cutting foreign DNA from bacteriophages (specific bacterial viruses) in both strands. Bacterial DNA is protected by a specific methylation of ''Eco''RI recognition sequences. | ||
== Reaction == | == Reaction == | ||
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''Eco''RI (E.C. 3.1.21.4) is a hydrolase and its substrate is a double-strand DNA molecule and two water molecules. For its catalytic activity, ''Eco''RI needs a cofactor, which is the divalent ion Mg<sup>2+</sup>. ''Eco''RI hydrolyses the phosphodiester bond between the guanylic and adenylic residues resulting in 5’-phosphate sticky ends, which are complementary. | ''Eco''RI (E.C. 3.1.21.4) is a hydrolase and its substrate is a double-strand DNA molecule and two water molecules. For its catalytic activity, ''Eco''RI needs a cofactor, which is the divalent ion Mg<sup>2+</sup>. ''Eco''RI hydrolyses the phosphodiester bond between the guanylic and adenylic residues resulting in 5’-phosphate sticky ends, which are complementary. | ||
== Structure == | == Structure == | ||
