Sandbox 206: Difference between revisions

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In this article we want to present you a new YFP (Yellow Fluorescent Protein) with improved maturation and reduced environmental sensitivity due to 5 mutation of the well characterized variant of the YFP that is to say the EYFP (Enhanced Yellow Fluorescent Protein). The PDB number of this molecule is 1MYW.

In this article we want to present you a new YFP (Yellow Fluorescent Protein) with improved maturation and reduced environmental sensitivity due to 5 mutation of the well characterized variant of the YFP that is to say the EYFP (Enhanced Yellow Fluorescent Protein). The PDB number of this molecule is 1MYW. <ref>http://www.rcsb.org/pdb/explore/explore.do?structureId=1MYW</ref>

== A new YFP called Venus (1MYW) ==

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==== The Chromophore ====

[[Image:Chromophore1MYW.gif |~~250px~~|left|thumb|]]

[[Image:Chromophore1MYW.gif |200px|left|thumb|caption=Chromophore of Venus]]

Chromophores almost always arise in one of two forms: conjugated pi systems and metal complexes. In our case, the chromophore is a conjugated pi-bond system. In this type of chromophores, the electrons jump between energy levels that are extended pi orbitals, created by a series of alternating single and double bonds, often in aromatic systems.<ref>http://www.chemguide.co.uk/analysis/uvvisible/theory.html#top</ref>.

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Venus can be used as a biomarker (YFP-tagged organelle marker for instance).

There is today a lot of application ~~but we~~ want to make you aware of one ~~of them~~ to see concretely what could be the role of this molecule in some search. ~~That is why, we~~ decided to take the example of the Team IGEM 2008 of the Albert Ludwigs University of Freibourg (Germany). [http://2008.igem.org/Team:Freiburg This is the site were you can found more informatrions]

There is today a lot of application in order to know how many or where a molecule is produce. We want to make you aware of one example to see concretely what could be the role of this molecule in some search. We decided to take the example of the Team IGEM 2008 of the Albert Ludwigs University of Freibourg (Germany). [http://2008.igem.org/Team:Freiburg This is the site were you can found more informatrions]

~~: '''Transfection and synthetic receptor activation : '''~~

~~''To show the localization of the constructs at the cell membrane transfection of the construct signal_peptide-Lipocalin-transmembrane_region-betaLactamase1-YFP was performed.~~

Figure 2_Transfection shows the model of the protein structure based on PDB files. Anti-fluorescein Anticalin (BBa_K157004) is the extracellular part of the construct. The transmembrane region is identical to that of the EGF-receptor erbb1 (BBa_K157002). Split beta-Lactamase (BBa_I757011), the intracellular part is fused to the yellow fluorescent protein to detect membrane localization.''

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Sandbox 206: Difference between revisions

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@@ Line 1: / Line 1: @@
 <Structure load='1myw' size='300' frame='true' align='left' caption='3D structure of Venus a new YFP with new propreties (1MYW) ''Cristalized withe a resolution of 2.20 Å'''/>
-In this article we want to present you a new YFP (Yellow Fluorescent Protein) with improved maturation and reduced environmental sensitivity due to 5 mutation of the well characterized variant of the YFP that is to say the EYFP (Enhanced Yellow Fluorescent Protein). The PDB number of this molecule is 1MYW.
+In this article we want to present you a new YFP (Yellow Fluorescent Protein) with improved maturation and reduced environmental sensitivity due to 5 mutation of the well characterized variant of the YFP that is to say the EYFP (Enhanced Yellow Fluorescent Protein). The PDB number of this molecule is 1MYW. <ref>http://www.rcsb.org/pdb/explore/explore.do?structureId=1MYW</ref>
 == A new YFP called Venus (1MYW) ==
@@ Line 18: / Line 18: @@
 ==== The Chromophore ====
-[[Image:Chromophore1MYW.gif |250px|left|thumb|]]
+[[Image:Chromophore1MYW.gif |200px|left|thumb|caption=Chromophore of Venus]]
 Chromophores almost always arise in one of two forms: conjugated pi systems and metal complexes. In our case, the chromophore is a conjugated pi-bond system. In this type of chromophores, the electrons jump between energy levels that are extended pi orbitals, created by a series of alternating single and double bonds, often in aromatic systems.<ref>http://www.chemguide.co.uk/analysis/uvvisible/theory.html#top</ref>.
@@ Line 41: / Line 41: @@
 Venus can be used as a biomarker (YFP-tagged organelle marker for instance).
-There is today a lot of application but we want to make you aware of one of them to see concretely what could be the role of this molecule in some search. That is why, we decided to take the example of the Team IGEM 2008 of the Albert Ludwigs University of Freibourg (Germany). [http://2008.igem.org/Team:Freiburg This is the site were you can found more informatrions]
+There is today a lot of application in order to know how many or where a molecule is produce. We want to make you aware of one example to see concretely what could be the role of this molecule in some search. We decided to take the example of the Team IGEM 2008 of the Albert Ludwigs University of Freibourg (Germany). [http://2008.igem.org/Team:Freiburg This is the site were you can found more informatrions]
-: '''Transfection and synthetic receptor activation : '''
-''To show the localization of the constructs at the cell membrane transfection of the construct signal_peptide-Lipocalin-transmembrane_region-betaLactamase1-YFP was performed.
-Figure 2_Transfection shows the model of the protein structure based on PDB files. Anti-fluorescein Anticalin (BBa_K157004) is the extracellular part of the construct. The transmembrane region is identical to that of the EGF-receptor erbb1 (BBa_K157002). Split beta-Lactamase (BBa_I757011), the intracellular part is fused to the yellow fluorescent protein to detect membrane localization.''
 ----