Sandbox 213: Difference between revisions
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= Mutation of Calmodulin gene and consequences = | = Mutation of Calmodulin gene and consequences = | ||
Calmodulin is an essential protein that is encoded in vertebrates by tree different genes (CaMI, CaMII and CaMIII) whereas it is encoded by a single gene in yeast and fungi (CMDI). Consequently the disruption of CMDI in yeast and fungi is lethal. However studies on ''Saccaromyces cerevisae'' had shown that vertebrate calmodulin can rescue yeast cells whose calmodulin has been deleted. That experience indicates that vertebrate calmodulin can functionally substitute for yeast calmodulin because ''Saccaromyces cerevisae'' calmodulin amino acids sequence is 60% identical to vertebrate Calmodulin. | Calmodulin is an essential protein that is encoded in vertebrates by tree different genes (CaMI, CaMII and CaMIII) whereas it is encoded by a single gene in yeast and fungi (CMDI). Consequently the disruption of CMDI in yeast and fungi is lethal. However studies on ''Saccaromyces cerevisae'' had shown that vertebrate calmodulin can rescue yeast cells whose calmodulin has been deleted. That experience indicates that vertebrate calmodulin can functionally substitute for yeast calmodulin because ''Saccaromyces cerevisae'' calmodulin amino acids sequence is 60% identical to vertebrate Calmodulin. | ||
= Calmodulin in laboratories = | |||
Because of widespread interest in calmodulin research many useful reagents are now available commercially. Purified calmodulin, anti-calmodulin, calmodulin-sepharose or -agarose and several calmodulin deficient target enzymes are available from major biochemical suppliers. For laboratories that plan extensive calmodulin research it may be more economically feasible to produce at least some of their reagents by various protocols such as calmodulin bioassay or calmodulin radioimmunoassay. | |||
= External Resources = | = External Resources = | ||
