Sandbox 206: Difference between revisions

Line 46:

We want to make you aware of one example to see concretely what could be the role of this molecule in some search. We decided to take the example of the Team IGEM 2008 of the Albert Ludwigs University of Freibourg (Germany). [http://2008.igem.org/Team:Freiburg This is the site were you can found more informatrions]

''~~Localization at the cell membrane~~

'''Abstract:'''

~~To show the localization of the constructs at the cell membrane transfection of the construct signal_peptide-Lipocalin-transmembrane_region-betaLactamase1-YFP was performed.~~

Figure 2_Transfection shows the model of the protein structure based on PDB files. Anti-fluorescein Anticalin (BBa_K157004) is the extracellular part of the construct. The transmembrane region is identical to that of the EGF-receptor erbb1 (BBa_K157002). Split beta-Lactamase (BBa_I757011), the intracellular part is fused to the yellow fluorescent protein to detect membrane localization.''

''Localization at the cell membrane''

''To show the localization of the constructs at the cell membrane transfection of the construct signal_peptide-Lipocalin-transmembrane_region-betaLactamase1-YFP was performed.

Figure 2_Transfection shows the model of the protein structure based on PDB files. Anti-fluorescein Anticalin (BBa_K157004) is the extracellular part of the construct. The transmembrane region is identical to that of the EGF-receptor erbb1 (BBa_K157002). Split beta-Lactamase (BBa_I757011), the intracellular part is fused to the yellow fluorescent protein to detect membrane localization.

''

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Sandbox 206: Difference between revisions

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@@ Line 46: / Line 46: @@
 We want to make you aware of one example to see concretely what could be the role of this molecule in some search. We decided to take the example of the Team IGEM 2008 of the Albert Ludwigs University of Freibourg (Germany). [http://2008.igem.org/Team:Freiburg This is the site were you can found more informatrions]
-''Localization at the cell membrane
+'''Abstract:'''
-To show the localization of the constructs at the cell membrane transfection of the construct signal_peptide-Lipocalin-transmembrane_region-betaLactamase1-YFP was performed.
-Figure 2_Transfection shows the model of the protein structure based on PDB files. Anti-fluorescein Anticalin (BBa_K157004) is the extracellular part of the construct. The transmembrane region is identical to that of the EGF-receptor erbb1 (BBa_K157002). Split beta-Lactamase (BBa_I757011), the intracellular part is fused to the yellow fluorescent protein to detect membrane localization.''
+''Localization at the cell membrane''
+''To show the localization of the constructs at the cell membrane transfection of the construct signal_peptide-Lipocalin-transmembrane_region-betaLactamase1-YFP was performed.
+Figure 2_Transfection shows the model of the protein structure based on PDB files. Anti-fluorescein Anticalin (BBa_K157004) is the extracellular part of the construct. The transmembrane region is identical to that of the EGF-receptor erbb1 (BBa_K157002). Split beta-Lactamase (BBa_I757011), the intracellular part is fused to the yellow fluorescent protein to detect membrane localization.
+''
 ----