2v1d: Difference between revisions
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==About this Structure== | ==About this Structure== | ||
2V1D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Fad Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V1D OCA]. | 2V1D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Fad+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V1D OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
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Revision as of 08:49, 3 February 2008
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STRUCTURAL BASIS OF LSD1-COREST SELECTIVITY IN HISTONE H3 RECOGNITION
Overview
Histone demethylase LSD1 regulates transcription by demethylating Lys(4), of histone H3. The crystal structure of the enzyme in complex with CoREST, and a substrate-like peptide inhibitor highlights an intricate network of, interactions and a folded conformation of the bound peptide. The core of, the peptide structure is formed by Arg(2), Gln(5), and Ser(10), which are, engaged in specific intramolecular H-bonds. Several charged side chains on, the surface of the substrate-binding pocket establish electrostatic, interactions with the peptide. The three-dimensional structure predicts, that methylated Lys(4) binds in a solvent inaccessible position in front, of the flavin cofactor. This geometry is fully consistent with the, demethylation reaction being catalyzed through a flavin-mediated oxidation, of the substrate amino-methyl group. These features dictate the exquisite, substrate specificity of LSD1 and provide a structural framework to, explain the fine tuning of its catalytic activity and the active role of, CoREST in substrate recognition.
About this Structure
2V1D is a Protein complex structure of sequences from Homo sapiens with FAD as ligand. Known structural/functional Site: AC1. Full crystallographic information is available from OCA.
Reference
Structural Basis of LSD1-CoREST Selectivity in Histone H3 Recognition., Forneris F, Binda C, Adamo A, Battaglioli E, Mattevi A, J Biol Chem. 2007 Jul 13;282(28):20070-4. Epub 2007 May 30. PMID:17537733
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Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Homo sapiens
- Protein complex
- Adamo, A.
- Battaglioli, E.
- Binda, C.
- Forneris, F.
- Mattevi, A.
- FAD
- Alternative splicing
- Amine oxidase
- Chromatin regulator
- Coiled coil
- Fad
- Flavin
- Histone demethylase
- Host-virus interaction
- Lsd1
- Nuclear protein
- Oxidoreductase
- Oxidoreductase/repressor complex chromatin remodelling
- Phosphorylation
- Repressor
- Transcription
- Transcription regulation
