3b6a: Difference between revisions

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[[Image:3b6a.jpg|left|200px]]<br /><applet load="3b6a" size="350" color="white" frame="true" align="right" spinBox="true"

caption="3b6a" />

caption="3b6a, resolution 3.05Å" />

''''''<br />

'''Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin'''<br />

==Overview==

Actinorhodin, an antibiotic produced by Streptomyces coelicolor, is, exported from the cell by the ActA efflux pump. actA is divergently, transcribed from actR, which encodes a TetR-like transcriptional, repressor. We showed previously that ActR represses transcription by, binding to an operator from the actA/actR intergenic region. Importantly, actinorhodin itself or various actinorhodin biosynthetic intermediates can, cause ActR to dissociate from its operator, leading to derepression. This, suggests that ActR may mediate timely self-resistance to an endogenously, produced antibiotic by responding to one of its biosynthetic precursors., Here, we report the structural basis for this precursor-mediated, derepression with crystal structures of homodimeric ActR by itself and in, complex with either actinorhodin or the actinorhodin biosynthetic, intermediate (S)-DNPA, [4-dihydro-9-hydroxy-1-methyl-10-oxo-3-H-naphtho-[2,3-c]-pyran-3-(S)-aceti, c acid]. The ligand-binding tunnel in each ActR monomer has a striking, hydrophilic/hydrophobic/hydrophilic arrangement of surface residues that, accommodate either one hexacyclic actinorhodin molecule or two, back-to-back tricyclic (S)-DNPA molecules. Moreover, our work also reveals, the strongest structural evidence to date that TetR-mediated antibiotic, resistance may have been acquired from an antibiotic-producer organism.

==About this Structure==

is a [http://en.wikipedia.org/wiki/~~Protein_complex Protein complex~~] structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA].

3B6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor] with <scene name='pdbligand=ZCT:'>ZCT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Zct+Binding+Site+For+Residue+A+1'>AC1</scene>, <scene name='pdbsite=AC2:Zct+Binding+Site+For+Residue+B+1'>AC2</scene>, <scene name='pdbsite=AC3:Zct+Binding+Site+For+Residue+C+1'>AC3</scene>, <scene name='pdbsite=AC4:Zct+Binding+Site+For+Residue+D+1'>AC4</scene>, <scene name='pdbsite=AC5:Zct+Binding+Site+For+Residue+E+1'>AC5</scene>, <scene name='pdbsite=AC6:Zct+Binding+Site+For+Residue+F+1'>AC6</scene>, <scene name='pdbsite=AC7:Zct+Binding+Site+For+Residue+G+1'>AC7</scene> and <scene name='pdbsite=AC8:Zct+Binding+Site+For+Residue+H+1'>AC8</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA].

[[Category: ~~Protein complex~~]]

==Reference==

Crystal Structures of the Streptomyces coelicolor TetR-Like Protein ActR Alone and in Complex with Actinorhodin or the Actinorhodin Biosynthetic Precursor (S)-DNPA., Willems AR, Tahlan K, Taguchi T, Zhang K, Lee ZZ, Ichinose K, Junop MS, Nodwell JR, J Mol Biol. 2008 Jan 4;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18207163 18207163]

[[Category: Single protein]]

[[Category: Streptomyces coelicolor]]

[[Category: Junop, M.S.]]

[[Category: Willems, A.R.]]

[[Category: ZCT]]

[[Category: actinorhodin]]

[[Category: dna-binding protein]]

[[Category: ligand]]

[[Category: tetr family]]

[[Category: transcription regulation]]

[[Category: transcriptional repressor]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 17:25:36 2008''

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@@ Line 1: / Line 1: @@
 [[Image:3b6a.jpg|left|200px]]<br /><applet load="3b6a" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="3b6a" />
+caption="3b6a, resolution 3.05&Aring;" />
-''''''<br />
+'''Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin'''<br />
+==Overview==
+Actinorhodin, an antibiotic produced by Streptomyces coelicolor, is, exported from the cell by the ActA efflux pump. actA is divergently, transcribed from actR, which encodes a TetR-like transcriptional, repressor. We showed previously that ActR represses transcription by, binding to an operator from the actA/actR intergenic region. Importantly, actinorhodin itself or various actinorhodin biosynthetic intermediates can, cause ActR to dissociate from its operator, leading to derepression. This, suggests that ActR may mediate timely self-resistance to an endogenously, produced antibiotic by responding to one of its biosynthetic precursors., Here, we report the structural basis for this precursor-mediated, derepression with crystal structures of homodimeric ActR by itself and in, complex with either actinorhodin or the actinorhodin biosynthetic, intermediate (S)-DNPA, [4-dihydro-9-hydroxy-1-methyl-10-oxo-3-H-naphtho-[2,3-c]-pyran-3-(S)-aceti, c acid]. The ligand-binding tunnel in each ActR monomer has a striking, hydrophilic/hydrophobic/hydrophilic arrangement of surface residues that, accommodate either one hexacyclic actinorhodin molecule or two, back-to-back tricyclic (S)-DNPA molecules. Moreover, our work also reveals, the strongest structural evidence to date that TetR-mediated antibiotic, resistance may have been acquired from an antibiotic-producer organism.
 ==About this Structure==
- is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA].
+B6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor] with <scene name='pdbligand=ZCT:'>ZCT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Zct+Binding+Site+For+Residue+A+1'>AC1</scene>, <scene name='pdbsite=AC2:Zct+Binding+Site+For+Residue+B+1'>AC2</scene>, <scene name='pdbsite=AC3:Zct+Binding+Site+For+Residue+C+1'>AC3</scene>, <scene name='pdbsite=AC4:Zct+Binding+Site+For+Residue+D+1'>AC4</scene>, <scene name='pdbsite=AC5:Zct+Binding+Site+For+Residue+E+1'>AC5</scene>, <scene name='pdbsite=AC6:Zct+Binding+Site+For+Residue+F+1'>AC6</scene>, <scene name='pdbsite=AC7:Zct+Binding+Site+For+Residue+G+1'>AC7</scene> and <scene name='pdbsite=AC8:Zct+Binding+Site+For+Residue+H+1'>AC8</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA].
-[[Category: Protein complex]]
+==Reference==
+Crystal Structures of the Streptomyces coelicolor TetR-Like Protein ActR Alone and in Complex with Actinorhodin or the Actinorhodin Biosynthetic Precursor (S)-DNPA., Willems AR, Tahlan K, Taguchi T, Zhang K, Lee ZZ, Ichinose K, Junop MS, Nodwell JR, J Mol Biol. 2008 Jan 4;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18207163 18207163]
+[[Category: Single protein]]
+[[Category: Streptomyces coelicolor]]
+[[Category: Junop, M.S.]]
+[[Category: Willems, A.R.]]
+[[Category: ZCT]]
+[[Category: actinorhodin]]
+[[Category: dna-binding protein]]
+[[Category: ligand]]
+[[Category: tetr family]]
+[[Category: transcription regulation]]
+[[Category: transcriptional repressor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb  6 15:37:43 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb  6 17:25:36 2008''