Sandbox 34: Difference between revisions

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=== Ligands ===

Papain binds ~~many~~ <scene name='Sandbox_34/~~Entire_protein_with_ligands~~/2'>methanol molecules</scene> via hydrogen ~~bonding~~. In addition to the methanol molecules, papain also has an interesting hydrogen bonding with water molecules. A refined crystal structure of papain, involving a restrained least-squares procedure at 1.65 A, with an estimated accuracy of 0.1 A, revealed that water forms something similar to a hydration shell around individual molecules of papain. The interaction of papain with these water molecules leads to less interaction between papain molecules, and contributes to the stability of the crystal structure of papain.<ref name="Structure" />

Papain binds both <scene name='Sandbox_34/Papainwithwaterandmtoh/1'>methanol and water molecules</scene> via hydrogen bonds. This solvent mixture of 62%, (w/w) <scene name='Sandbox_34/Entire_protein_with_ligands/2'>methanol</scene> in <scene name='Sandbox_34/Papainwithwateronly/1'>water</scene> was used in order to obtain a C-type crystal. In addition to the methanol molecules, papain also has an interesting hydrogen bonding with water molecules. A refined crystal structure of papain, involving a restrained least-squares procedure at 1.65 A, with an estimated accuracy of 0.1 A, revealed that water forms something similar to a hydration shell around individual molecules of papain. The interaction of papain with these water molecules leads to less interaction between papain molecules, and contributes to the stability of the crystal structure of papain.<ref name="Structure" />

=== Specificity ===

Sandbox 34: Difference between revisions

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@@ Line 30: / Line 30: @@
 === Ligands ===
-Papain binds many <scene name='Sandbox_34/Entire_protein_with_ligands/2'>methanol molecules</scene> via hydrogen bonding. In addition to the methanol molecules, papain also has an interesting hydrogen bonding with water molecules. A refined crystal structure of papain, involving a restrained least-squares procedure at 1.65 A, with an estimated accuracy of 0.1 A, revealed that water forms something similar to a hydration shell around individual molecules of papain. The interaction of papain with these water molecules leads to less interaction between papain molecules, and contributes to the stability of the crystal structure of papain.<ref name="Structure" />
+Papain binds both <scene name='Sandbox_34/Papainwithwaterandmtoh/1'>methanol and water molecules</scene> via hydrogen bonds. This solvent mixture of 62%, (w/w) <scene name='Sandbox_34/Entire_protein_with_ligands/2'>methanol</scene> in <scene name='Sandbox_34/Papainwithwateronly/1'>water</scene> was used in order to obtain a C-type crystal. In addition to the methanol molecules, papain also has an interesting hydrogen bonding with water molecules. A refined crystal structure of papain, involving a restrained least-squares procedure at 1.65 A, with an estimated accuracy of 0.1 A, revealed that water forms something similar to a hydration shell around individual molecules of papain. The interaction of papain with these water molecules leads to less interaction between papain molecules, and contributes to the stability of the crystal structure of papain.<ref name="Structure" />
 === Specificity ===