Sandbox 34: Difference between revisions

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=== Solvent Interactions ===
=== Solvent Interactions ===


Papain binds both <scene name='Sandbox_34/Papainwithwaterandmtoh/1'>methanol and water molecules</scene> via hydrogen bonds. This solvent mixture of 62%, (w/w) <scene name='Sandbox_34/Entire_protein_with_ligands/3'>methanol</scene> in <scene name='Sandbox_34/Papainwithwateronly/1'>water</scene> was used in order to obtain a C-type crystal, which is . In addition to the methanol molecules, papain also has an interesting hydrogen bonding with water molecules. A refined crystal structure of papain, involving a restrained least-squares procedure at 1.65 A, with an estimated accuracy of 0.1 A, revealed that water forms something similar to a hydration shell around individual molecules of papain. The interaction of papain with these water molecules leads to less interaction between papain molecules, and contributes to the stability of the crystal structure of papain.<ref name="Structure" />
Papain binds both <scene name='Sandbox_34/Papainwithwaterandmtoh/1'>methanol and water molecules</scene> via hydrogen bonding. This solvent mixture of 62%, (w/w) methanol to water was used in order to obtain a C-type crystal. Papain has an interesting method of utilizing hydrogen bonding with <scene name='Sandbox_34/Papainwithwateronly/1'>water molecules</scene>. A refined crystal structure of papain, involving a restrained least-squares procedure at 1.65 A, with an estimated accuracy of 0.1 A, revealed that water forms something similar to a hydration shell around individual molecules of papain. The interaction of papain with these water molecules leads to less interaction between papain molecules, and contributes to the stability of the crystal structure of papain. The water molecules also form hydrogen bonds within the <scene name='Sandbox_34/Papainwithwaterandmtoh/2'>active site</scene>, providing even more stability for the crystalline structure.<ref name="Structure" />  




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