Sandbox 47: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
|||
| Line 12: | Line 12: | ||
== '''Function''' == | == '''Function''' == | ||
Most lipases act at a specific position on the glycerol backbone of the lipid substrate.<ref>"Lipase". Wikipedia: The Free Encyclopedia. 6 Nov 2011 [http://en.wikipedia.org/wiki/Lipase]</ref> Pancreatic lipase catalyzes the hydrolysis of triacylglycerols at their 1 and 3 positions to form 1,2-diacylglycerols and 2-acylglycerols together with the Na+ and K+ salts of fatty acids. The enzymatic activity of pancreatic lipase increases when it contacts the lipid-water interface. Binding to the lipid-water interface requries mixed micelles of phosphatidylcholine and bile acids as well as colipase.<ref>Voet D, Voet JG, Pratt CW. "Fundamentals of Biochemistry: Life at the Molecular Level" John Wiley and Sons, Inc: New Jersey, 2008..</ref> | Most lipases act at a specific position on the glycerol backbone of the lipid substrate.<ref>"Lipase". Wikipedia: The Free Encyclopedia. 6 Nov 2011 [http://en.wikipedia.org/wiki/Lipase]</ref> Pancreatic lipase catalyzes the hydrolysis of triacylglycerols at their 1 and 3 positions to form 1,2-diacylglycerols and 2-acylglycerols together with the Na+ and K+ salts of fatty acids. The enzymatic activity of pancreatic lipase increases when it contacts the lipid-water interface. Binding to the lipid-water interface requries mixed micelles of phosphatidylcholine and bile acids as well as colipase.<ref>Voet D, Voet JG, Pratt CW. "Fundamentals of Biochemistry: Life at the Molecular Level" John Wiley and Sons, Inc: New Jersey, 2008..</ref> | ||
[[Image:Hydrolysis.gif|200px|right|thumb| Lipase-catalyzed hydrolysis of lipids. Notice the catalytic triad of Ser-152, Asp-176, and His-263 that constitute the active site.<ref>http://www.pnas.org/content/101/16/5716.full</ref>]] | |||
== '''Colipase''' == | == '''Colipase''' == | ||
