Sandbox 49: Difference between revisions

The mechanism of action for pancreatic lipase can be broken down into 4 main steps. In the first step His 263 deprotonates Ser 152 that in turn attacks the carboxy carbon of triacylglyceride substrate, it is a nucleophilic addition reaction. In the second step of the reaction the oxyanion hole collapses resulting in the elimination of the diacylglycerol product, that deprotonates His 263 and acylates Ser 152 as a result. In the third step His 263 deprotonates water, which in turn attacks the carboxyl carbon of the acylated Ser 152 in another nucleophilic addition reaction. And in the last step the oxyanion hole collapses again resulting in the elimination of the carboxylate product and Ser 152 which then deprotonates His 263.