User:Marvin O'Neal/VlsE: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 18: | Line 18: | ||
Throughout the course of the disease IR6 produces a strong antibody response that can be identified from early to late phases, and which lasts for months to years following treatment. Applications in diagnostic testing have been identified as a result of this strong immune response and IR6’s relative invariability across strains (4, 5). A C6 ELISA test has been developed which uses a 26-mer synthetic peptide with the IR6 sequence. Results show 99% specificity and 100% precision with high sensitivity. In fact, OspA vaccination does not influence C6 specificity; therefore, C6 ELISA tests are valuable diagnostic tools for patients vaccinated against OspA. The CDC currently recommends a two-step test incorporating first an ELISA followed by a Western blot to eliminate false positives. Therefore, this one-step ELISA test presents an economical and more accurate improvement over the current two-step model (4). <br /> | Throughout the course of the disease IR6 produces a strong antibody response that can be identified from early to late phases, and which lasts for months to years following treatment. Applications in diagnostic testing have been identified as a result of this strong immune response and IR6’s relative invariability across strains (4, 5). A C6 ELISA test has been developed which uses a 26-mer synthetic peptide with the IR6 sequence. Results show 99% specificity and 100% precision with high sensitivity. In fact, OspA vaccination does not influence C6 specificity; therefore, C6 ELISA tests are valuable diagnostic tools for patients vaccinated against OspA. The [http://www.cdc.gov/lyme/healthcare/clinician_twotier.html CDC] ([http://www.cdc.gov/about/grand-rounds/archives/2011/pdfs/PHGRLymeMay2011.pdf (pdf)] currently recommends a two-step test incorporating first an ELISA followed by a Western blot to eliminate false positives. Therefore, this one-step ELISA test presents an economical and more accurate improvement over the current two-step model (4). <br /> | ||
