The interaction of FOXP3 with NFAT1 and the FOXP3-NFAT1 target sequences found in IL-2 has been investigated extensively. The <scene name='Forkhead_Box_Protein_3/Foxp3_monomer/1'>Forkhead domain of FOXP3</scene> appears to form a <scene name='Forkhead_Box_Protein_3/Foxp3_dimer/1'>domain swapped dimer</scene> with a <scene name='Forkhead_Box_Protein_3/Nfat_dimer/2'>dimerized rel homology region (RHR) of NFAT1</scene> and two unique <scene name='Forkhead_Box_Protein_3/Oligonucleotides/2'>oligonucleotides</scene>, each containing distinct FOXP sites.<ref name="Chen">PMID: 21458306</ref>
The interaction of FOXP3 with NFAT1 and the FOXP3-NFAT1 target sequences found in IL-2 has been investigated extensively. The <scene name='Forkhead_Box_Protein_3/Foxp3_monomer/1'>Forkhead domain of FOXP3</scene> appears to form a <scene name='Forkhead_Box_Protein_3/Foxp3_dimer/1'>domain swapped dimer</scene> with a <scene name='Forkhead_Box_Protein_3/Nfat_dimer/2'>dimerized rel homology region (RHR) of NFAT1</scene> and two unique <scene name='Forkhead_Box_Protein_3/Oligonucleotides/2'>oligonucleotides</scene>, each containing distinct FOXP sites.<ref name="Chen">PMID: 21458306</ref>
Each domain-swapped dimer of FOXP3 makes extensive interactions with NFAT1 involving residues Thr359, Asn361, His365, Glu399, and Glu401 of FOXP3, among others, which were critical in the [[FOXP2]]-NFAT1 interaction.<ref name="Chen"/>
<ref name="Chen"/>
<ref name="Chen"/>
Revision as of 21:18, 28 April 2012
Structure of the Forkhead domain of FOXP3 bound to NFAT and IL2 Promoter Oligonucleotide (3qrf)
Forkhead Box Protein 3 (FOXP3) is a member of the Forkhead transcription factor family. It is highly expressed in regulatory T (Treg) cells, a subset of CD4+ T cells that play a critical role in suppressing immune responses, especially those mediated by autoreactive T cells.[1] FOXP3 upregulates a number of genes like Cd25 and Ctla4 and represses other genes like IL-2 and Ptpn22.[2] As with many transcription factors, it cooperates with a number of transcription factor partners to regulate gene expression, including NFAT1, which participates in the inducible expression of cytokine genes like IL-2, IL-4, and TNFα in T cells.[3] A number of mutations to FOXP3 are known to result in a severe autoimmune disease known as IPEX (immune dysregulation, polyendocriopthy, enteropathy, X-linked). As FOXP3 is found on the X-chromosome, mutations to FOXP3 typically only display deleterious phenotypic traits in males, resulting in lymphocyte infiltration and wide spread inflammation in inphants.[4] A similar pathology is also found in mice who carry nonsense mutations in the FOXP3 locus. These mutant mice are known as scurfy mice. The targeted elimination of FOXP3+ CD4+ Tregs in adult mice has similar autoimmune dysfunction.[5] Further, ectopic expression of FOXP3 in peripheral CD4+CD25- T cells equips these T cells with the ability to suppress the proliferation and effector functions of autoreactive T cells in vivo.[6]
Each domain-swapped dimer of FOXP3 makes extensive interactions with NFAT1 involving residues Thr359, Asn361, His365, Glu399, and Glu401 of FOXP3, among others, which were critical in the FOXP2-NFAT1 interaction.[7]
Talk about alignment with FOXP2 and fix the morph.
↑Josefowicz SZ, Rudensky A. Control of regulatory T cell lineage commitment and maintenance. Immunity. 2009 May;30(5):616-25. PMID:19464984 doi:10.1016/j.immuni.2009.04.009
↑Zheng Y, Josefowicz SZ, Kas A, Chu TT, Gavin MA, Rudensky AY. Genome-wide analysis of Foxp3 target genes in developing and mature regulatory T cells. Nature. 2007 Feb 22;445(7130):936-40. Epub 2007 Jan 21. PMID:17237761 doi:10.1038/nature05563
↑Rudra D, Egawa T, Chong MM, Treuting P, Littman DR, Rudensky AY. Runx-CBFbeta complexes control expression of the transcription factor Foxp3 in regulatory T cells. Nat Immunol. 2009 Nov;10(11):1170-7. Epub 2009 Sep 20. PMID:19767756 doi:10.1038/ni.1795
↑Bennett CL, Christie J, Ramsdell F, Brunkow ME, Ferguson PJ, Whitesell L, Kelly TE, Saulsbury FT, Chance PF, Ochs HD. The immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) is caused by mutations of FOXP3. Nat Genet. 2001 Jan;27(1):20-1. PMID:11137993 doi:10.1038/83713
↑Williams LM, Rudensky AY. Maintenance of the Foxp3-dependent developmental program in mature regulatory T cells requires continued expression of Foxp3. Nat Immunol. 2007 Mar;8(3):277-84. Epub 2007 Jan 14. PMID:17220892 doi:10.1038/ni1437
↑Fontenot JD, Gavin MA, Rudensky AY. Foxp3 programs the development and function of CD4+CD25+ regulatory T cells. Nat Immunol. 2003 Apr;4(4):330-6. Epub 2003 Mar 3. PMID:12612578 doi:10.1038/ni904
↑ 7.07.17.2Bandukwala HS, Wu Y, Feurer M, Chen Y, Barbosa B, Ghosh S, Stroud JC, Benoist C, Mathis D, Rao A, Chen L. Structure of a Domain-Swapped FOXP3 Dimer on DNA and Its Function in Regulatory T Cells. Immunity. 2011 Mar 30. PMID:21458306 doi:10.1016/j.immuni.2011.02.017
References
Proteopedia Page Contributors and Editors (what is this?)
