Sandbox Reserved 470: Difference between revisions
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'''Other roles:''' | '''Other roles:''' | ||
The importance of GAPDH in transcription was discovered by Zheng ''et. al''. in 2003. It was found that OCA's transcriptional coactivator complex contains GAPDH, which moves between the cytosol and nucleus and may link the metabolic state to gene transcription. | *The importance of GAPDH in transcription was discovered by Zheng ''et. al''. in 2003. It was found that OCA's transcriptional coactivator complex contains GAPDH, which moves between the cytosol and nucleus and may link the metabolic state to gene transcription. | ||
In 2005 the initiation of apoptosis was shown to be mediated by GAPDH by Hara ''et. al.'' when it was found to bind to DNA like it does in transcription activation. | *In 2005 the initiation of apoptosis was shown to be mediated by GAPDH by Hara ''et. al.'' when it was found to bind to DNA like it does in transcription activation. | ||
GAPDH was also found to be involved in ER to Golgi transport because it is recruited by rab2 to vesicular-tubular clusters of the endoplasmic reticulum where it helps form COP 1 vesicles. | GAPDH was also found to be involved in ER to Golgi transport because it is recruited by rab2 to vesicular-tubular clusters of the endoplasmic reticulum where it helps form COP 1 vesicles. | ||
It was also found that GAPDH is plays a role in certain neruodegenerative disorders, as is able to find stretches which are encoded by the gene's CAG repeats and bind to the gene products formed from disorders such as [http://en.wikipedia.org/wiki/Huntington's_disease Huntington's disease], [http://en.wikipedia.org/wiki/Alzheimer's_disease Alzheimer’s disease], [http://en.wikipedia.org/wiki/Parkinson's_disease Parkinson’s disease] and [http://en.wikipedia.org/wiki/Machado-Joseph_disease Machado-Joseph disease]. | *It was also found that GAPDH is plays a role in certain neruodegenerative disorders, as is able to find stretches which are encoded by the gene's CAG repeats and bind to the gene products formed from disorders such as [http://en.wikipedia.org/wiki/Huntington's_disease Huntington's disease], [http://en.wikipedia.org/wiki/Alzheimer's_disease Alzheimer’s disease], [http://en.wikipedia.org/wiki/Parkinson's_disease Parkinson’s disease] and [http://en.wikipedia.org/wiki/Machado-Joseph_disease Machado-Joseph disease]. | ||
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'''Use in the lab:''' | '''Use in the lab:''' | ||
Overall, GAPDH is a “housekeeping gene” and is found in high levels in tissues and cells so it is commonly used in biological research as a loading control in [http://en.wikipedia.org/wiki/Western_Blot western blot] and [http://en.wikipedia.org/wiki/RT-PCR RT-PCR], but it has to be carefully controlled because under specific conditions it can have various regulation. | [[Image:GAPDH western blot.jpg]] | ||
*Overall, GAPDH is a “housekeeping gene” and is found in high levels in tissues and cells so it is commonly used in biological research as a loading control in [http://en.wikipedia.org/wiki/Western_Blot western blot] and [http://en.wikipedia.org/wiki/RT-PCR RT-PCR], but it has to be carefully controlled because under specific conditions it can have various regulation. | |||
