Sandbox Reserved 470: Difference between revisions
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'''Use in the lab:''' | '''Use in the lab:''' | ||
*Overall, GAPDH is a “housekeeping gene” and is found in high levels in tissues and cells so it is commonly used in biological research as a loading control in [http://en.wikipedia.org/wiki/Western_Blot western blot] and [http://en.wikipedia.org/wiki/RT-PCR RT-PCR], but it has to be carefully controlled because under specific conditions it can have various regulation. | *Overall, GAPDH is a “housekeeping gene” and is found in high levels in tissues and cells so it is commonly used in biological research as a loading control in [http://en.wikipedia.org/wiki/Western_Blot western blot] and [http://en.wikipedia.org/wiki/RT-PCR RT-PCR], but it has to be carefully controlled because under specific conditions it can have various regulation. | ||
[[Image:GAPDH western blot.jpg|350 px]] | |||
[[Image:RT-PCR GAPDH.jpg|200 px]] | |||
