Sandbox Reserved 496: Difference between revisions

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The remaining two metalloclusters (both A-clusters) are located in the two α subunits of the CODH/ACS protein. As the active site of ACS activity in each α subunit, the A-clusters are composed of a [Fe4S4] center bridged to a binuclear site. There is some debate regarding the metal ions present in the binuclear site; either <scene name='Sandbox_Reserved_496/Alpha_a_cluster/1'>both A-clusters are [Fe4S4]-Cu-Ni</scene> <ref name="Cu"/> or one A-cluster is [Fe4S4]-Ni-Ni while the other is [Fe4S4]-Zn-Ni <ref name="Zn"/>. The [Fe4S4] center is coordinated by Cys506, Cys509, Cys518, and Cys528 with Cys509 also forming the bridge between the [Fe4S4] and the [http://en.wikipedia.org/wiki/Proximal#Proximal_and_distal proximal] metal ion. Furthermore, the distal metal ion is coordinated by Cys595, Gly596, and Cys597with Cys595 and Cys597 also connecting the distal and the proximal metal ions. Only the [Fe4S4]-Ni-Ni A-cluster is in an open conformation, the other two site types with Cu or Zn as the proximal ion are in closed form.

The remaining two metalloclusters (both A-clusters) are located in the two α subunits of the CODH/ACS protein. As the active site of ACS activity in each α subunit, the A-clusters are composed of a [Fe4S4] center bridged to a binuclear site. There is some debate regarding the metal ions present in the binuclear site; either <scene name='Sandbox_Reserved_496/Alpha_a_cluster/1'>both A-clusters are [Fe4S4]-Cu-Ni</scene> <ref name="Cu"/> or one A-cluster is <scene name='Sandbox_Reserved_496/A_cluster_ni_ni/1'>[Fe4S4]-Ni-Ni</scene> while the other is [Fe4S4]-Zn-Ni <ref name="Zn"/>. The [Fe4S4] center is coordinated by Cys506, Cys509, Cys518, and Cys528 with Cys509 also forming the bridge between the [Fe4S4] and the [http://en.wikipedia.org/wiki/Proximal#Proximal_and_distal proximal] metal ion. Furthermore, the distal metal ion is coordinated by Cys595, Gly596, and Cys597with Cys595 and Cys597 also connecting the distal and the proximal metal ions. Only the [Fe4S4]-Ni-Ni A-cluster is in an open conformation, the other two site types with Cu or Zn as the proximal ion are in closed form.

Sandbox Reserved 496: Difference between revisions

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Revision as of 23:20, 2 May 2012 view source Rachel Slivka (talk \| contribs) 49 edits No edit summary ← Older edit		Revision as of 23:38, 2 May 2012 view source Rachel Slivka (talk \| contribs) 49 edits No edit summary Newer edit →
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	The remaining two metalloclusters (both A-clusters) are located in the two α subunits of the CODH/ACS protein. As the active site of ACS activity in each α subunit, the A-clusters are composed of a [Fe4S4] center bridged to a binuclear site. There is some debate regarding the metal ions present in the binuclear site; either <scene name='Sandbox_Reserved_496/Alpha_a_cluster/1'>both A-clusters are [Fe4S4]-Cu-Ni</scene> <ref name="Cu"/> or one A-cluster is [Fe4S4]-Ni-Ni while the other is [Fe4S4]-Zn-Ni <ref name="Zn"/>. The [Fe4S4] center is coordinated by Cys506, Cys509, Cys518, and Cys528 with Cys509 also forming the bridge between the [Fe4S4] and the [http://en.wikipedia.org/wiki/Proximal#Proximal_and_distal proximal] metal ion. Furthermore, the distal metal ion is coordinated by Cys595, Gly596, and Cys597with Cys595 and Cys597 also connecting the distal and the proximal metal ions. Only the [Fe4S4]-Ni-Ni A-cluster is in an open conformation, the other two site types with Cu or Zn as the proximal ion are in closed form.		The remaining two metalloclusters (both A-clusters) are located in the two α subunits of the CODH/ACS protein. As the active site of ACS activity in each α subunit, the A-clusters are composed of a [Fe4S4] center bridged to a binuclear site. There is some debate regarding the metal ions present in the binuclear site; either <scene name='Sandbox_Reserved_496/Alpha_a_cluster/1'>both A-clusters are [Fe4S4]-Cu-Ni</scene> <ref name="Cu"/> or one A-cluster is <scene name='Sandbox_Reserved_496/A_cluster_ni_ni/1'>[Fe4S4]-Ni-Ni</scene> while the other is [Fe4S4]-Zn-Ni <ref name="Zn"/>. The [Fe4S4] center is coordinated by Cys506, Cys509, Cys518, and Cys528 with Cys509 also forming the bridge between the [Fe4S4] and the [http://en.wikipedia.org/wiki/Proximal#Proximal_and_distal proximal] metal ion. Furthermore, the distal metal ion is coordinated by Cys595, Gly596, and Cys597with Cys595 and Cys597 also connecting the distal and the proximal metal ions. Only the [Fe4S4]-Ni-Ni A-cluster is in an open conformation, the other two site types with Cu or Zn as the proximal ion are in closed form.