Sandbox Reserved 470: Difference between revisions
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==Structure== | ==Structure== | ||
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*While GAPDH possesses four cysteine residues, its <scene name='Sandbox_Reserved_470/Active_site_gapdh/1'>active site</scene> is located around <scene name='Sandbox_Reserved_470/Cysteine_active_site/1'>Cysteine 149</scene>. | *While GAPDH possesses four cysteine residues, its <scene name='Sandbox_Reserved_470/Active_site_gapdh/1'>active site</scene> is located around <scene name='Sandbox_Reserved_470/Cysteine_active_site/1'>Cysteine 149</scene>, which is responsible for inhibition of glycolysis and fermentation by iodoacetic acid. '''<ref>''' Boyer, Paul D.. "The Enzymes - Paul D. Boyer - Google Books." Google Books. N.p., n.d. Web. 1 May 2012. '''[<http://books.google.com/books?id=r6SRFsxYFYwC&pg=PA20&lpg=PA20&dq=GAPDH+cysteine+149&source=bl&ots=0CWwdduzGB&sig=Lb8PtWq7EGdwyvdAnFI_rHYG3tU&hl=en&sa=X&ei=2PyhT-WtHJSy8QTjtsnTCA&ved=0CDMQ6AEwAg#v=onepage&q=GAPDH%20cysteine%20149&f=false>]'''. '''</ref>''' | ||
*GAPDH has two domains, a GAPDH-like, C-terminal domain, and an NAD Binding Domain. | *GAPDH has two domains, a GAPDH-like, C-terminal domain, and an NAD Binding Domain. | ||
:The <scene name='Sandbox_Reserved_470/Nad_binding_domain/3'>NAD (Nucleotide) Binding Domain </scene> is an Alpha Beta 3-Layer(α-β-α) Sandwich, including amino acids 1-138 and 301-340 on chains O and Q . Its [http://en.wikipedia.org/wiki/CATH CATH] reports a Rossmann fold and a classification as an [http://en.wikipedia.org/wiki/Oxidoreductase oxidoreductase]. | :The <scene name='Sandbox_Reserved_470/Nad_binding_domain/3'>NAD (Nucleotide) Binding Domain </scene> is an Alpha Beta 3-Layer(α-β-α) Sandwich, including amino acids 1-138 and 301-340 on chains O and Q . Its [http://en.wikipedia.org/wiki/CATH CATH] reports a Rossmann fold and a classification as an [http://en.wikipedia.org/wiki/Oxidoreductase oxidoreductase]. | ||
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*Depending on the environment, including variations in temperature and acidity, GAPDH can have different structural qualities and physical properties. In some cases, it is observed as a twinned structure with an α/β barrel. | *Depending on the environment, including variations in temperature and acidity, GAPDH can have different structural qualities and physical properties. In some cases, it is observed as a twinned structure with an α/β barrel. | ||
[[Image:Cysteine_active_site.jpeg]] | |||
==Importance== | ==Importance== | ||
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==References== | ==References== | ||
<references/> | <references/> | ||
#↑Boyer, Paul D.. "The Enzymes - Paul D. Boyer - Google Books." Google Books. N.p., n.d. Web. 1 May 2012. '''<ref>''' Boyer, Paul D.. "The Enzymes - Paul D. Boyer - Google Books." Google Books. N.p., n.d. Web. 1 May 2012. '''[<http://books.google.com/books?id=r6SRFsxYFYwC&pg=PA20&lpg=PA20&dq=GAPDH+cysteine+149&source=bl&ots=0CWwdduzGB&sig=Lb8PtWq7EGdwyvdAnFI_rHYG3tU&hl=en&sa=X&ei=2PyhT-WtHJSy8QTjtsnTCA&ved=0CDMQ6AEwAg#v=onepage&q=GAPDH%20cysteine%20149&f=false>]'''. '''</ref>''' | |||
#↑"GAPDH glyceraldehyde-3-phosphate dehydrogenase [Homo sapiens] - Gene - NCBI." National Center for Biotechnology Information. N.p., n.d. Web. 1 May 2012. '''<ref>'''"GAPDH glyceraldehyde-3-phosphate dehydrogenase [Homo sapiens] - Gene - NCBI." National Center for Biotechnology Information. N.p., n.d. Web. 1 May 2012. '''[<http://www.ncbi.nlm.nih.gov/gene/2597>]'''. '''<ref/>''' | #↑"GAPDH glyceraldehyde-3-phosphate dehydrogenase [Homo sapiens] - Gene - NCBI." National Center for Biotechnology Information. N.p., n.d. Web. 1 May 2012. '''<ref>'''"GAPDH glyceraldehyde-3-phosphate dehydrogenase [Homo sapiens] - Gene - NCBI." National Center for Biotechnology Information. N.p., n.d. Web. 1 May 2012. '''[<http://www.ncbi.nlm.nih.gov/gene/2597>]'''. '''<ref/>''' | ||
#↑"Glyceraldehyde 3-phosphate dehydrogenase." Stanford University. N.p., n.d. Web. 22 Apr. 2012. '''<ref>'''"Glyceraldehyde 3-phosphate dehydrogenase." Stanford University. N.p., n.d. Web. 22 Apr. 2012. '''[<http://www.stanford.edu/~mmogri/bio/gapdh/structure.html>]'''. '''</ref>''' | #↑"Glyceraldehyde 3-phosphate dehydrogenase." Stanford University. N.p., n.d. Web. 22 Apr. 2012. '''<ref>'''"Glyceraldehyde 3-phosphate dehydrogenase." Stanford University. N.p., n.d. Web. 22 Apr. 2012. '''[<http://www.stanford.edu/~mmogri/bio/gapdh/structure.html>]'''. '''</ref>''' | ||
