User:Marvin O'Neal/OspA: Difference between revisions
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<scene name='Studio:G2SecL03/Ospa-loop1/1' target="OspA-manip">Loop 1</scene>, (residues 203-220), is important in showing variation amongst the different strains of <i>Borrelia</i> as well as being optimally conformed for binding without steric hindrance. <scene name='Studio:G2SecL03/Ospa-loop2/1' target="OspA-manip">Loop 2</scene> (residues 224-233) and <scene name='Studio:G2SecL03/Ospa-loop3/1' target="OspA-manip">Loop 3</scene> (residues 246-257) are more strongly conserved than Loop 1 but also help to show some variation amongst strains. The LA-2 Fab antibody readily recognizes OspA from <i>B. burgdorferi</i>, but does not recognize that from <i>B. afzelii</i> or <i>B. garinii</i>. | <scene name='Studio:G2SecL03/Ospa-loop1/1' target="OspA-manip">Loop 1</scene>, (residues 203-220), is important in showing variation amongst the different strains of <i>Borrelia</i> as well as being optimally conformed for binding without steric hindrance. <scene name='Studio:G2SecL03/Ospa-loop2/1' target="OspA-manip">Loop 2</scene> (residues 224-233) and <scene name='Studio:G2SecL03/Ospa-loop3/1' target="OspA-manip">Loop 3</scene> (residues 246-257) are more strongly conserved than Loop 1 but also help to show some variation amongst strains. The LA-2 Fab antibody readily recognizes OspA from <i>B. burgdorferi</i>, but does not recognize that from <i>B. afzelii</i> or <i>B. garinii</i>. | ||
Between | Between <i>B. burgdorferi</i> and <i>B. afzelii</i> genetic sequences are generally invariant, but two residues change between the species: <scene name='Studio:G2SecL03/Ospa-ala208/1' target="OspA-manip">Alanine (Ala) 208</scene> in <i>B. burgdorferi</i> is a Glutamine (Gln) in <i>B. afzelii</i>, and <scene name='Studio:G2SecL03/Ospa-asn251/1' target="OspA-manip">Asparagine (Asn) 251</scene> in <i>B. burgdorferi</i> is an Alanine (Ala) in <i>B. afzelii</i>. <i>B. garinii</i> has more variation and in addition to the previous two differences, having at least one more difference, where <scene name='Studio:G2SecL03/Ospa-ala215/1' target="OspA-manip">Alanine (Ala) 215</scene> in <i>B. burgdorferi</i> is a Lysine (Lys), and sometimes also has a deletion at <i>B. burgdorferi</i>’s Alanine 208. LA-2 and OspA of <i>B. burgdorferi</i> form a tight interface when binding, and the longer Glutamine (Gln) sidechain found in <i>B. afzelii</i> and <i>B. garinii</i> is more difficult to accommodate, causing less binding. A chimera that was weakly recognized by LA-2 was made with parts of loop 1 from <i>B. burgdorferi</i>, and loops 2 and 3 from <i>B. garinii</i>.<ref name="ding">PMID: 11183781</ref> Recently, a different kind of chimera has been made which combined the proximal region of <i>B. burgdorferi</i> and distal region of <i>B. afzelii</i>, and was able to successfully protect mice from both species. 8 | ||
</p> | </p> | ||
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<ul> | <ul> | ||
<li><scene name='Studio:G2SecL03/Ospa-3residues-r/2' target="OspA-manip">Display R-groups</scene> of Ala208, Ala215 and Asn251</li> | <li><scene name='Studio:G2SecL03/Ospa-3residues-r/2' target="OspA-manip">Display R-groups</scene> of Ala208, Ala215 and Asn251</li> | ||
<li><scene name='Studio:G2SecL03/Ospa-ala208/1' target="OspA-manip">Alanine (Ala) 208</scene></li> | |||
<li><scene name='Studio:G2SecL03/Ospa-ala215/1' target="OspA-manip">Alanine (Ala) 215</scene></li> | |||
<li><scene name='Studio:G2SecL03/Ospa-asn251/1' target="OspA-manip">Asparagine (Asn) 251</scene></li> | |||
</ul> | </ul> | ||
</li> | </li> | ||
