P53-DNA Recognition: Difference between revisions

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Protein side chains and base pairs form direct contacts in the major groove among which the <scene name='Sandbox_Reserved_170/Arg280_contact/5'>contact between Arg280 and the guanine of the core element</scene> contributes most to binding specificity. This highly specific readout is due to the <scene name='Sandbox_Reserved_170/Arg280_contact/4'>bidentate hydrogen bond formed between Arg280 and guanine</scene>. As a result of this '''base readout''' the identity of the G/C base pairs in the CWWG core elements is the most conserved position in p53 response elements (Figure 5).

Another important contact is formed with the <scene name='Sandbox_Reserved_170/Lys_120/3'>Lys120 residue from the L1 loop of the protein</scene>. Lys120 is biologically very important because acetylation of this residue is known to trigger the apoptotic response.

Another important contact is formed with the <scene name='Sandbox_Reserved_170/Lys_120/3'>Lys120 residue from the L1 loop of the protein</scene>. Lys120 is biologically very important because acetylation of this residue is known to trigger the apoptotic response of p53.

===DNA Backbone Contact===

Another arginine residue, <scene name='Sandbox_Reserved_170/Arg273/2'>Arg273 contacts the phosphodiester backbone</scene> and seems to be important for p53-DNA binding.Moreover, Arg273 is the second most common missense mutation in human cancer (Figure 2).

Another arginine residue, <scene name='Sandbox_Reserved_170/Arg273/2'>Arg273 contacts the phosphodiester backbone</scene> and seems to be important for p53-DNA binding. Moreover, Arg273 is the second most common missense mutation in human cancer (Figure 2).

[[Image:Kitayner-etal-Figure7.jpg|thumb|right|400px|Figure 7: DNA shape readout of narrow minor groove regions with enhanced electrostatic potential by Arg248<ref name='kitayner'/>. Nature Publishing Group has provided permission for usage of this figure.]]

P53-DNA Recognition: Difference between revisions

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@@ Line 45: / Line 45: @@
 Protein side chains and base pairs form direct contacts in the major groove among which the <scene name='Sandbox_Reserved_170/Arg280_contact/5'>contact between Arg280 and the guanine of the core element</scene> contributes most to binding specificity. This highly specific readout is due to the <scene name='Sandbox_Reserved_170/Arg280_contact/4'>bidentate hydrogen bond formed between Arg280 and guanine</scene>. As a result of this '''base readout''' the identity of the G/C base pairs in the CWWG core elements is the most conserved position in p53 response elements (Figure 5).
-Another important contact is formed with the <scene name='Sandbox_Reserved_170/Lys_120/3'>Lys120 residue from the L1 loop of the protein</scene>. Lys120 is biologically very important because acetylation of this residue is known to trigger the apoptotic response.
+Another important contact is formed with the <scene name='Sandbox_Reserved_170/Lys_120/3'>Lys120 residue from the L1 loop of the protein</scene>. Lys120 is biologically very important because acetylation of this residue is known to trigger the apoptotic response of p53.
 ===DNA Backbone Contact===
-Another arginine residue, <scene name='Sandbox_Reserved_170/Arg273/2'>Arg273 contacts the phosphodiester backbone</scene> and seems to be important for p53-DNA binding.Moreover, Arg273 is the second most common missense mutation in human cancer (Figure 2).
+Another arginine residue, <scene name='Sandbox_Reserved_170/Arg273/2'>Arg273 contacts the phosphodiester backbone</scene> and seems to be important for p53-DNA binding. Moreover, Arg273 is the second most common missense mutation in human cancer (Figure 2).
 [[Image:Kitayner-etal-Figure7.jpg|thumb|right|400px|Figure 7: DNA shape readout of narrow minor groove regions with enhanced electrostatic potential by Arg248<ref name='kitayner'/>. Nature Publishing Group has provided permission for usage of this figure.]]