P53-DNA Recognition: Difference between revisions

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=Further Reading=

Hoogsteen base pairs have previously been found in protein-DNA complexes but usually associated with drastic deformations of the DNA. Only in one case of a homeodomain protein, a Hoogsteen base pair was identified in undistorted B-DNA<ref name="wolberger">Aishima J, Gitti RK, Noah JE, Gan HH, Schlick T, Wolberger C. A Hoogsteen base pair embedded in undistorted B-DNA. Nucleic Acids Res. 2002;30(23):5244-52.</ref>.

Hoogsteen base pairs have previously been found in protein-DNA complexes but usually associated with drastic deformations of the DNA. Only in one case of a homeodomain protein, a Hoogsteen base pair was identified in undistorted B-DNA<ref name="wolberger">Aishima J, Gitti RK, Noah JE, Gan HH, Schlick T, Wolberger C. A Hoogsteen base pair embedded in undistorted B-DNA. Nucleic Acids Res. 2002;30(23):5244-52. [http://www.ncbi.nlm.nih.gov/pubmed/12466549 PMID:12466549].</ref>.

As for p53-DNA recognition, Hoogsteen base pairs are not present in the complex with a different DNA sequence <ref name="chen">Chen Y, Dey R, Chen L. Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer. Structure. 2010;18(2):246-56.</ref> but the DNA undergoes a different deformation not observed in the complex with Hoogsteen base pairing. Since transient Hoogsteen base pairs have been detected in naked DNA with preferences for CA and TA dincucleotides<ref name="hashimi">Nikolova EN, Kim E, Wise AA, O'Brien PJ, Andricioaei I, Al-Hashimi HM. Transient Hoogsteen base pairs in canonical duplex DNA. Nature. 2011;470(7335):498-502.</ref>, the observation of the base pairing variant in p53-DNA complexes indicates a sequence-speific feature of the response element either recognized or stabilized by p53.

P53-DNA Recognition: Difference between revisions

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 =Further Reading=
-Hoogsteen base pairs have previously been found in protein-DNA complexes but usually associated with drastic deformations of the DNA. Only in one case of a homeodomain protein, a Hoogsteen base pair was identified in undistorted B-DNA<ref name="wolberger">Aishima J, Gitti RK, Noah JE, Gan HH, Schlick T, Wolberger C. A Hoogsteen base pair embedded in undistorted B-DNA. Nucleic Acids Res. 2002;30(23):5244-52.</ref>.
+Hoogsteen base pairs have previously been found in protein-DNA complexes but usually associated with drastic deformations of the DNA. Only in one case of a homeodomain protein, a Hoogsteen base pair was identified in undistorted B-DNA<ref name="wolberger">Aishima J, Gitti RK, Noah JE, Gan HH, Schlick T, Wolberger C. A Hoogsteen base pair embedded in undistorted B-DNA. Nucleic Acids Res. 2002;30(23):5244-52. [http://www.ncbi.nlm.nih.gov/pubmed/12466549 PMID:12466549].</ref>.
 As for p53-DNA recognition, Hoogsteen base pairs are not present in the complex with a different DNA sequence <ref name="chen">Chen Y, Dey R, Chen L. Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer. Structure. 2010;18(2):246-56.</ref> but the DNA undergoes a different deformation not observed in the complex with Hoogsteen base pairing. Since transient Hoogsteen base pairs have been detected in naked DNA with preferences for CA and TA dincucleotides<ref name="hashimi">Nikolova EN, Kim E, Wise AA, O'Brien PJ, Andricioaei I, Al-Hashimi HM. Transient Hoogsteen base pairs in canonical duplex DNA. Nature. 2011;470(7335):498-502.</ref>, the observation of the base pairing variant in p53-DNA complexes indicates a sequence-speific feature of the response element either recognized or stabilized by p53.