1a4k: Difference between revisions
New page: left|200px<br /> <applet load="1a4k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a4k, resolution 2.4Å" /> '''DIELS ALDER CATALYTI... |
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[[Image:1a4k.gif|left|200px]]<br /> | [[Image:1a4k.gif|left|200px]]<br /><applet load="1a4k" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1a4k, resolution 2.4Å" /> | caption="1a4k, resolution 2.4Å" /> | ||
'''DIELS ALDER CATALYTIC ANTIBODY WITH TRANSITION STATE ANALOGUE'''<br /> | '''DIELS ALDER CATALYTIC ANTIBODY WITH TRANSITION STATE ANALOGUE'''<br /> | ||
==Overview== | ==Overview== | ||
The three-dimensional structure of an antibody (39-A11) that catalyzes a | The three-dimensional structure of an antibody (39-A11) that catalyzes a Diels-Alder reaction has been determined. The structure suggests that the antibody catalyzes this pericyclic reaction through a combination of packing and hydrogen-bonding interactions that control the relative geometries of the bound substrates and electronic distribution in the dienophile. A single somatic mutation, serine-91 of the light chain to valine, is largely responsible for the increase in affinity and catalytic activity of the affinity-matured antibody. Structural and functional studies of the germ-line precursor suggest that 39-A11 and related antibodies derive from a family of germ-line genes that have been selected throughout evolution for the ability of the encoded proteins to form a polyspecific combining site. Germ line-encoded antibodies of this type, which can rapidly evolve into high-affinity receptors for a broad range of structures, may help to expand the binding potential associated with the structural diversity of the primary antibody repertoire. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1A4K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with CD and FRA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1A4K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=CD:'>CD</scene> and <scene name='pdbligand=FRA:'>FRA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A4K OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Romesburg, F | [[Category: Romesburg, F E.]] | ||
[[Category: Schultz, P | [[Category: Schultz, P G.]] | ||
[[Category: Spiller, B | [[Category: Spiller, B W.]] | ||
[[Category: Stevens, R | [[Category: Stevens, R C.]] | ||
[[Category: CD]] | [[Category: CD]] | ||
[[Category: FRA]] | [[Category: FRA]] | ||
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[[Category: immunoglobulin]] | [[Category: immunoglobulin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:40:47 2008'' | ||
Revision as of 09:40, 21 February 2008
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DIELS ALDER CATALYTIC ANTIBODY WITH TRANSITION STATE ANALOGUE
Overview
The three-dimensional structure of an antibody (39-A11) that catalyzes a Diels-Alder reaction has been determined. The structure suggests that the antibody catalyzes this pericyclic reaction through a combination of packing and hydrogen-bonding interactions that control the relative geometries of the bound substrates and electronic distribution in the dienophile. A single somatic mutation, serine-91 of the light chain to valine, is largely responsible for the increase in affinity and catalytic activity of the affinity-matured antibody. Structural and functional studies of the germ-line precursor suggest that 39-A11 and related antibodies derive from a family of germ-line genes that have been selected throughout evolution for the ability of the encoded proteins to form a polyspecific combining site. Germ line-encoded antibodies of this type, which can rapidly evolve into high-affinity receptors for a broad range of structures, may help to expand the binding potential associated with the structural diversity of the primary antibody repertoire.
Disease
Known disease associated with this structure: Kappa light chain deficiency OMIM:[147200]
About this Structure
1A4K is a Single protein structure of sequence from Mus musculus with CD and FRA as ligands. Full crystallographic information is available from OCA.
Reference
Immunological origins of binding and catalysis in a Diels-Alderase antibody., Romesberg FE, Spiller B, Schultz PG, Stevens RC, Science. 1998 Mar 20;279(5358):1929-33. PMID:9506942
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