1a9m: Difference between revisions

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[[Image:1a9m.gif|left|200px]]<br />

[[Image:1a9m.gif|left|200px]]<br /><applet load="1a9m" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1a9m" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1a9m, resolution 2.3Å" />

'''G48H MUTANT OF HIV-1 PROTEASE IN COMPLEX WITH A PEPTIDIC INHIBITOR U-89360E'''<br />

==Overview==

The crystal structure of human immunodeficiency virus type 1 (HIV-1), protease mutant G48H with peptidic inhibitor U-89360E is described., Comparison with wild-type protease-inhibitor complex shows that mutation, of flap residue 48 to histidine allows stabilizing van der Waals contacts, between the side chains of His48 and Phe53 as well as between His48 and, the P2' and P3' inhibitor subsites. The flap region is less mobile than in, the wild-type enzyme. A model of saquinavir-resistant mutant protease G48V, in complex with saquinavir predicts interactions similar to those found in, the G48H crystal. Energetic calculations confirm the similarity of the, His48 and Val48 interactions.

The crystal structure of human immunodeficiency virus type 1 (HIV-1) protease mutant G48H with peptidic inhibitor U-89360E is described. Comparison with wild-type protease-inhibitor complex shows that mutation of flap residue 48 to histidine allows stabilizing van der Waals contacts between the side chains of His48 and Phe53 as well as between His48 and the P2' and P3' inhibitor subsites. The flap region is less mobile than in the wild-type enzyme. A model of saquinavir-resistant mutant protease G48V in complex with saquinavir predicts interactions similar to those found in the G48H crystal. Energetic calculations confirm the similarity of the His48 and Val48 interactions.

==About this Structure==

1A9M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus Human immunodeficiency virus] with U0E as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1A9M OCA].

1A9M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus Human immunodeficiency virus] with <scene name='pdbligand=U0E:'>U0E</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A9M OCA].

==Reference==

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[[Category: Single protein]]

[[Category: Foundling, S.]]

[[Category: Hartsuck, J.A.]]

[[Category: Hartsuck, J A.]]

[[Category: Hong, L.]]

[[Category: Tang, J.]]

[[Category: Zhang, X.J.]]

[[Category: Zhang, X J.]]

[[Category: U0E]]

[[Category: aspartyl protease]]

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[[Category: mutation]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on Thu ~~Nov 8 13~~:49:~~44 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:42:20 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1a9m.gif|left|200px]]<br />
+[[Image:1a9m.gif|left|200px]]<br /><applet load="1a9m" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1a9m" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1a9m, resolution 2.3&Aring;" />
 '''G48H MUTANT OF HIV-1 PROTEASE IN COMPLEX WITH A PEPTIDIC INHIBITOR U-89360E'''<br />
 ==Overview==
-The crystal structure of human immunodeficiency virus type 1 (HIV-1), protease mutant G48H with peptidic inhibitor U-89360E is described., Comparison with wild-type protease-inhibitor complex shows that mutation, of flap residue 48 to histidine allows stabilizing van der Waals contacts, between the side chains of His48 and Phe53 as well as between His48 and, the P2' and P3' inhibitor subsites. The flap region is less mobile than in, the wild-type enzyme. A model of saquinavir-resistant mutant protease G48V, in complex with saquinavir predicts interactions similar to those found in, the G48H crystal. Energetic calculations confirm the similarity of the, His48 and Val48 interactions.
+The crystal structure of human immunodeficiency virus type 1 (HIV-1) protease mutant G48H with peptidic inhibitor U-89360E is described. Comparison with wild-type protease-inhibitor complex shows that mutation of flap residue 48 to histidine allows stabilizing van der Waals contacts between the side chains of His48 and Phe53 as well as between His48 and the P2' and P3' inhibitor subsites. The flap region is less mobile than in the wild-type enzyme. A model of saquinavir-resistant mutant protease G48V in complex with saquinavir predicts interactions similar to those found in the G48H crystal. Energetic calculations confirm the similarity of the His48 and Val48 interactions.
 ==About this Structure==
-A9M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus Human immunodeficiency virus] with U0E as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A9M OCA].
+A9M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus Human immunodeficiency virus] with <scene name='pdbligand=U0E:'>U0E</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A9M OCA].
 ==Reference==
@@ Line 15: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Foundling, S.]]
-[[Category: Hartsuck, J.A.]]
+[[Category: Hartsuck, J A.]]
 [[Category: Hong, L.]]
 [[Category: Tang, J.]]
-[[Category: Zhang, X.J.]]
+[[Category: Zhang, X J.]]
 [[Category: U0E]]
 [[Category: aspartyl protease]]
@@ Line 24: / Line 23: @@
 [[Category: mutation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov  8 13:49:44 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:42:20 2008''