1b2p: Difference between revisions
New page: left|200px<br /><applet load="1b2p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b2p, resolution 1.7Å" /> '''NATIVE MANNOSE-SPECIF... |
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[[Image:1b2p.gif|left|200px]]<br /><applet load="1b2p" size=" | [[Image:1b2p.gif|left|200px]]<br /><applet load="1b2p" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1b2p, resolution 1.7Å" /> | caption="1b2p, resolution 1.7Å" /> | ||
'''NATIVE MANNOSE-SPECIFIC BULB LECTIN FROM SCILLA CAMPANULATA (BLUEBELL) AT 1.7 ANGSTROMS RESOLUTION'''<br /> | '''NATIVE MANNOSE-SPECIFIC BULB LECTIN FROM SCILLA CAMPANULATA (BLUEBELL) AT 1.7 ANGSTROMS RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
The X-ray crystal structure of native Scilla campanulata agglutinin, a | The X-ray crystal structure of native Scilla campanulata agglutinin, a mannose-specific lectin from bluebell bulbs and a member of the Liliaceae family, has been determined by molecular replacement and refined to an R value of 0.186 at 1.7 A resolution. The lectin crystallizes in space group P21212 with unit-cell parameters a = 70. 42, b = 92.95, c = 46.64 A. The unit cell contains eight protein molecules of Mr = 13143 Da (119 amino-acid residues). The asymmetric unit comprises two chemically identical molecules, A and B, related by a non-crystallographic twofold axis perpendicular to c. This dimer further associates by crystallographic twofold symmetry to form a tetramer. The fold of the polypeptide backbone closely resembles that found in the lectins from Galanthus nivalis (snowdrop) and Hippeastrum (amaryllis) and contains a threefold symmetric beta-prism made up of three antiparallel four-stranded beta-sheets. Each of the four-stranded beta-sheets (I, II and III) possesses a potential saccharide-binding site containing conserved residues; however, site II has two mutations relative to sites I and III which may prevent ligation at this site. Our study provides the first accurate and detailed description of a native (unligated) structure from this superfamily of mannose-specific bulb lectins and will allow comparisons with a number of lectin-saccharide complexes which have already been determined or are currently under investigation. | ||
==About this Structure== | ==About this Structure== | ||
1B2P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hyacinthoides_hispanica Hyacinthoides hispanica]. Full crystallographic information is available from [http:// | 1B2P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hyacinthoides_hispanica Hyacinthoides hispanica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2P OCA]. | ||
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Hyacinthoides hispanica]] | [[Category: Hyacinthoides hispanica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Allen, A | [[Category: Allen, A K.]] | ||
[[Category: Damme, E | [[Category: Damme, E J.M Van.]] | ||
[[Category: Peumans, W | [[Category: Peumans, W J.]] | ||
[[Category: Reynolds, C | [[Category: Reynolds, C D.]] | ||
[[Category: Rizkallah, P | [[Category: Rizkallah, P J.]] | ||
[[Category: Wood, S | [[Category: Wood, S D.]] | ||
[[Category: Wright, L | [[Category: Wright, L M.]] | ||
[[Category: aglutinin]] | [[Category: aglutinin]] | ||
[[Category: bluebell bulbs]] | [[Category: bluebell bulbs]] | ||
| Line 26: | Line 26: | ||
[[Category: protein- carbohydrate interactions]] | [[Category: protein- carbohydrate interactions]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:50:56 2008'' | ||
Revision as of 09:51, 21 February 2008
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NATIVE MANNOSE-SPECIFIC BULB LECTIN FROM SCILLA CAMPANULATA (BLUEBELL) AT 1.7 ANGSTROMS RESOLUTION
Overview
The X-ray crystal structure of native Scilla campanulata agglutinin, a mannose-specific lectin from bluebell bulbs and a member of the Liliaceae family, has been determined by molecular replacement and refined to an R value of 0.186 at 1.7 A resolution. The lectin crystallizes in space group P21212 with unit-cell parameters a = 70. 42, b = 92.95, c = 46.64 A. The unit cell contains eight protein molecules of Mr = 13143 Da (119 amino-acid residues). The asymmetric unit comprises two chemically identical molecules, A and B, related by a non-crystallographic twofold axis perpendicular to c. This dimer further associates by crystallographic twofold symmetry to form a tetramer. The fold of the polypeptide backbone closely resembles that found in the lectins from Galanthus nivalis (snowdrop) and Hippeastrum (amaryllis) and contains a threefold symmetric beta-prism made up of three antiparallel four-stranded beta-sheets. Each of the four-stranded beta-sheets (I, II and III) possesses a potential saccharide-binding site containing conserved residues; however, site II has two mutations relative to sites I and III which may prevent ligation at this site. Our study provides the first accurate and detailed description of a native (unligated) structure from this superfamily of mannose-specific bulb lectins and will allow comparisons with a number of lectin-saccharide complexes which have already been determined or are currently under investigation.
About this Structure
1B2P is a Single protein structure of sequence from Hyacinthoides hispanica. Full crystallographic information is available from OCA.
Reference
Structure of the native (unligated) mannose-specific bulb lectin from Scilla campanulata (bluebell) at 1.7 A resolution., Wood SD, Wright LM, Reynolds CD, Rizkallah PJ, Allen AK, Peumans WJ, Van Damme EJ, Acta Crystallogr D Biol Crystallogr. 1999 Jul;55(Pt 7):1264-72. PMID:10393293
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