1b3p: Difference between revisions
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New page: left|200px<br /><applet load="1b3p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b3p" /> '''5'-D(*GP*GP*AP*GP*GP*AP*T)-3''''<br /> ==Ov... |
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[[Image:1b3p.gif|left|200px]]<br /><applet load="1b3p" size=" | [[Image:1b3p.gif|left|200px]]<br /><applet load="1b3p" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1b3p" /> | caption="1b3p" /> | ||
'''5'-D(*GP*GP*AP*GP*GP*AP*T)-3''''<br /> | '''5'-D(*GP*GP*AP*GP*GP*AP*T)-3''''<br /> | ||
==Overview== | ==Overview== | ||
BACKGROUND: Triplet repeat sequences are of considerable biological | BACKGROUND: Triplet repeat sequences are of considerable biological importance as the expansion of such tandem arrays can lead to the onset of a range of human diseases. Such sequences can self-pair via mismatch alignments to form higher order structures that have the potential to cause replication blocks, followed by strand slippage and sequence expansion. The all-purine d(GGA)n triplet repeat sequence is of particular interest because purines can align via G.G, A.A and G.A mismatch formation. RESULTS: We have solved the structure of the uniformly 13C,15N-labeled d(G1-G2-A3-G4-G5-A6-T7) sequence in 10 mM Na+ solution. This sequence adopts a novel twofold-symmetric duplex fold where interlocked V-shaped arrowhead motifs are aligned solely via interstrand G1.G4, G2.G5 and A3.A6 mismatch formation. The tip of the arrowhead motif is centered about the p-A3-p step, and symmetry-related local parallel-stranded duplex domains are formed by the G1-G2-A3 and G4-G5-A6 segments of partner strands. CONCLUSIONS: The purine-rich (GGA)n triplet repeat sequence is dispersed throughout the eukaryotic genome. Several features of the arrowhead duplex motif for the (GGA)2 triplet repeat provide a unique scaffold for molecular recognition. These include the large localized bend in the sugar-phosphate backbones, the segmental parallel-stranded alignment of strands and the exposure of the Watson-Crick edges of several mismatched bases. | ||
==About this Structure== | ==About this Structure== | ||
1B3P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | 1B3P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3P OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Bouaziz, S.]] | [[Category: Bouaziz, S.]] | ||
[[Category: Jones, R | [[Category: Jones, R A.]] | ||
[[Category: Kettani, A.]] | [[Category: Kettani, A.]] | ||
[[Category: Majumdar, A.]] | [[Category: Majumdar, A.]] | ||
[[Category: Patel, D | [[Category: Patel, D J.]] | ||
[[Category: Skripkin, E.]] | [[Category: Skripkin, E.]] | ||
[[Category: Wang, W.]] | [[Category: Wang, W.]] | ||
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[[Category: 15n-labeled dna]] | [[Category: 15n-labeled dna]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:51:07 2008'' | ||
