1b4y: Difference between revisions
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'''STRUCTURE AND MECHANISM OF FORMATION OF THE H-Y5 ISOMER OF AN INTRAMOLECULAR DNA TRIPLE HELIX.'''<br /> | '''STRUCTURE AND MECHANISM OF FORMATION OF THE H-Y5 ISOMER OF AN INTRAMOLECULAR DNA TRIPLE HELIX.'''<br /> | ||
==Overview== | ==Overview== | ||
H-DNA, thought to play a regulatory role in transcription, exists in two | H-DNA, thought to play a regulatory role in transcription, exists in two isomeric forms, H-y3 and H-y5. We present the first solution structure of a DNA fragment representing the H-y5 fold. The structure shows the H-y5 triple helix, and for the first time how in an H-DNA isomer the purine strand extension interacts with the triplex loop. It gives direct insight into the mechanism of H-DNA formation, and explains a host of biochemical and biophysical data on the relative stability of the H-DNA isomers. In addition, the observed interaction of the purine strand extension and the triplex loop provides new clues to the design of clamp-type triple helix-forming oligonucleotides. | ||
==About this Structure== | ==About this Structure== | ||
1B4Y is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | 1B4Y is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B4Y OCA]. | ||
==Reference== | ==Reference== | ||
Structure and mechanism of formation of the H-y5 isomer of an intramolecular DNA triple helix., van Dongen MJ, Doreleijers JF, van der Marel GA, van Boom JH, Hilbers CW, Wijmenga SS, Nat Struct Biol. 1999 Sep;6(9):854-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10467098 10467098] | Structure and mechanism of formation of the H-y5 isomer of an intramolecular DNA triple helix., van Dongen MJ, Doreleijers JF, van der Marel GA, van Boom JH, Hilbers CW, Wijmenga SS, Nat Struct Biol. 1999 Sep;6(9):854-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10467098 10467098] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Boom, J | [[Category: Boom, J H.Van.]] | ||
[[Category: Dongen, M | [[Category: Dongen, M J.P Van.]] | ||
[[Category: Doreleijers, J | [[Category: Doreleijers, J F.]] | ||
[[Category: Hilbers, C | [[Category: Hilbers, C W.]] | ||
[[Category: Marel, G | [[Category: Marel, G A.Van Der.]] | ||
[[Category: Wijmenga, S | [[Category: Wijmenga, S S.]] | ||
[[Category: dna]] | [[Category: dna]] | ||
[[Category: h-dna]] | [[Category: h-dna]] | ||
[[Category: triple helix]] | [[Category: triple helix]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:51:33 2008'' | ||
Revision as of 09:51, 21 February 2008
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STRUCTURE AND MECHANISM OF FORMATION OF THE H-Y5 ISOMER OF AN INTRAMOLECULAR DNA TRIPLE HELIX.
Overview
H-DNA, thought to play a regulatory role in transcription, exists in two isomeric forms, H-y3 and H-y5. We present the first solution structure of a DNA fragment representing the H-y5 fold. The structure shows the H-y5 triple helix, and for the first time how in an H-DNA isomer the purine strand extension interacts with the triplex loop. It gives direct insight into the mechanism of H-DNA formation, and explains a host of biochemical and biophysical data on the relative stability of the H-DNA isomers. In addition, the observed interaction of the purine strand extension and the triplex loop provides new clues to the design of clamp-type triple helix-forming oligonucleotides.
About this Structure
1B4Y is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure and mechanism of formation of the H-y5 isomer of an intramolecular DNA triple helix., van Dongen MJ, Doreleijers JF, van der Marel GA, van Boom JH, Hilbers CW, Wijmenga SS, Nat Struct Biol. 1999 Sep;6(9):854-9. PMID:10467098
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