1bg3: Difference between revisions
New page: left|200px<br /><applet load="1bg3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bg3, resolution 2.8Å" /> '''RAT BRAIN HEXOKINASE ... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1bg3.gif|left|200px]]<br /><applet load="1bg3" size=" | [[Image:1bg3.gif|left|200px]]<br /><applet load="1bg3" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1bg3, resolution 2.8Å" /> | caption="1bg3, resolution 2.8Å" /> | ||
'''RAT BRAIN HEXOKINASE TYPE I COMPLEX WITH GLUCOSE AND INHIBITOR GLUCOSE-6-PHOSPHATE'''<br /> | '''RAT BRAIN HEXOKINASE TYPE I COMPLEX WITH GLUCOSE AND INHIBITOR GLUCOSE-6-PHOSPHATE'''<br /> | ||
==Overview== | ==Overview== | ||
We have determined the structures of the glucose-6-phosphate | We have determined the structures of the glucose-6-phosphate (G6P)-inhibitable 100,000 Mr Type I hexokinase from rat and the G6P-sensitive 50,000 Mr hexokinase from Schistosoma mansoni at a resolution of 2.8 and 2.6 A respectively. The structures define the glucose and G6P binding sites in these enzymes, suggest the mechanisms of intradomain G6P inhibition and activity loss in the Type I hexokinase N-terminal half, and reveal the structure of the membrane targeting motif that integrates the Type I hexokinase into the outer mitochondrial membrane. | ||
==About this Structure== | ==About this Structure== | ||
1BG3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with GLC, G6P and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hexokinase Hexokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.1 2.7.1.1] Full crystallographic information is available from [http:// | 1BG3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=G6P:'>G6P</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hexokinase Hexokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.1 2.7.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BG3 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Garavito, R | [[Category: Garavito, R M.]] | ||
[[Category: Mulichak, A | [[Category: Mulichak, A M.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
[[Category: G6P]] | [[Category: G6P]] | ||
| Line 22: | Line 22: | ||
[[Category: phosphotransferase]] | [[Category: phosphotransferase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:52 2008'' | ||
Revision as of 09:54, 21 February 2008
|
RAT BRAIN HEXOKINASE TYPE I COMPLEX WITH GLUCOSE AND INHIBITOR GLUCOSE-6-PHOSPHATE
Overview
We have determined the structures of the glucose-6-phosphate (G6P)-inhibitable 100,000 Mr Type I hexokinase from rat and the G6P-sensitive 50,000 Mr hexokinase from Schistosoma mansoni at a resolution of 2.8 and 2.6 A respectively. The structures define the glucose and G6P binding sites in these enzymes, suggest the mechanisms of intradomain G6P inhibition and activity loss in the Type I hexokinase N-terminal half, and reveal the structure of the membrane targeting motif that integrates the Type I hexokinase into the outer mitochondrial membrane.
About this Structure
1BG3 is a Single protein structure of sequence from Rattus norvegicus with GLC, G6P and CA as ligands. Active as Hexokinase, with EC number 2.7.1.1 Full crystallographic information is available from OCA.
Reference
The structure of mammalian hexokinase-1., Mulichak AM, Wilson JE, Padmanabhan K, Garavito RM, Nat Struct Biol. 1998 Jul;5(7):555-60. PMID:9665168
Page seeded by OCA on Thu Feb 21 11:54:52 2008
