1bg7: Difference between revisions

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[[Image:1bg7.gif|left|200px]]<br /><applet load="1bg7" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1bg7.gif|left|200px]]<br /><applet load="1bg7" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1bg7, resolution 1.85Å" />

'''LOCALIZED UNFOLDING AT THE JUNCTION OF THREE FERRITIN SUBUNITS. A MECHANISM FOR IRON RELEASE?'''<br />

==Overview==

How and where iron exits from ferritin for cellular use is unknown., Twenty-four protein subunits create a cavity in ferritin where iron is, concentrated >10(11)-fold as a mineral. Proline substitution for conserved, leucine 134 (L134P) allowed normal assembly but increased iron exit rates., X-ray crystallography of H-L134P ferritin revealed localized unfolding at, the 3-fold axis, also iron entry sites, consistent with shared use sites, for iron exit and entry. The junction of three ferritin subunits appears, to be a dynamic aperture with a "shutter" that cytoplasmic factors might, open or close to regulate iron release in vivo.

How and where iron exits from ferritin for cellular use is unknown. Twenty-four protein subunits create a cavity in ferritin where iron is concentrated >10(11)-fold as a mineral. Proline substitution for conserved leucine 134 (L134P) allowed normal assembly but increased iron exit rates. X-ray crystallography of H-L134P ferritin revealed localized unfolding at the 3-fold axis, also iron entry sites, consistent with shared use sites for iron exit and entry. The junction of three ferritin subunits appears to be a dynamic aperture with a "shutter" that cytoplasmic factors might open or close to regulate iron release in vivo.

==About this Structure==

1BG7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1BG7 OCA].

1BG7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BG7 OCA].

==Reference==

Line 13:

[[Category: Rana catesbeiana]]

[[Category: Single protein]]

[[Category: Allewell, N.M.]]

[[Category: Allewell, N M.]]

[[Category: Ha, Y.]]

[[Category: Shi, D.]]

[[Category: Takagi, H.]]

[[Category: Theil, E.C.]]

[[Category: Theil, E C.]]

[[Category: CA]]

[[Category: ferritin]]

[[Category: iron storage]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 11:37:~~34 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:56 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1bg7.gif|left|200px]]<br /><applet load="1bg7" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1bg7.gif|left|200px]]<br /><applet load="1bg7" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1bg7, resolution 1.85&Aring;" />
 '''LOCALIZED UNFOLDING AT THE JUNCTION OF THREE FERRITIN SUBUNITS. A MECHANISM FOR IRON RELEASE?'''<br />
 ==Overview==
-How and where iron exits from ferritin for cellular use is unknown., Twenty-four protein subunits create a cavity in ferritin where iron is, concentrated &gt;10(11)-fold as a mineral. Proline substitution for conserved, leucine 134 (L134P) allowed normal assembly but increased iron exit rates., X-ray crystallography of H-L134P ferritin revealed localized unfolding at, the 3-fold axis, also iron entry sites, consistent with shared use sites, for iron exit and entry. The junction of three ferritin subunits appears, to be a dynamic aperture with a "shutter" that cytoplasmic factors might, open or close to regulate iron release in vivo.
+How and where iron exits from ferritin for cellular use is unknown. Twenty-four protein subunits create a cavity in ferritin where iron is concentrated &gt;10(11)-fold as a mineral. Proline substitution for conserved leucine 134 (L134P) allowed normal assembly but increased iron exit rates. X-ray crystallography of H-L134P ferritin revealed localized unfolding at the 3-fold axis, also iron entry sites, consistent with shared use sites for iron exit and entry. The junction of three ferritin subunits appears to be a dynamic aperture with a "shutter" that cytoplasmic factors might open or close to regulate iron release in vivo.
 ==About this Structure==
-BG7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BG7 OCA].
+BG7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BG7 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Rana catesbeiana]]
 [[Category: Single protein]]
-[[Category: Allewell, N.M.]]
+[[Category: Allewell, N M.]]
 [[Category: Ha, Y.]]
 [[Category: Shi, D.]]
 [[Category: Takagi, H.]]
-[[Category: Theil, E.C.]]
+[[Category: Theil, E C.]]
 [[Category: CA]]
 [[Category: ferritin]]
 [[Category: iron storage]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:37:34 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:56 2008''