1bk6: Difference between revisions

Line 1:

[[Image:1bk6.gif|left|200px]]<br /><applet load="1bk6" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1bk6.gif|left|200px]]<br /><applet load="1bk6" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1bk6, resolution 2.8Å" />

'''KARYOPHERIN ALPHA (YEAST) + SV40 T ANTIGEN NLS'''<br />

==Overview==

Selective nuclear import is mediated by nuclear localization signals, (NLSs) and cognate transport factors known as karyopherins or importins., Karyopherin alpha recognizes "classical" monopartite and bipartite NLSs., We report the crystal structure of a 50 kDa fragment of the 60 kDa yeast, karyopherin alpha, in the absence and presence of a monopartite NLS, peptide at 2.2 A and 2.8 A resolution, respectively. The structure shows a, tandem array of ten armadillo repeats, organized in a right-handed, superhelix of helices. Binding of the NLS peptide occurs at two sites, within a helical surface groove that is lined by conserved residues. The, structure reveals the determinants of NLS specificity and suggests a model, for the recognition of bipartite NLSs.

Selective nuclear import is mediated by nuclear localization signals (NLSs) and cognate transport factors known as karyopherins or importins. Karyopherin alpha recognizes "classical" monopartite and bipartite NLSs. We report the crystal structure of a 50 kDa fragment of the 60 kDa yeast karyopherin alpha, in the absence and presence of a monopartite NLS peptide at 2.2 A and 2.8 A resolution, respectively. The structure shows a tandem array of ten armadillo repeats, organized in a right-handed superhelix of helices. Binding of the NLS peptide occurs at two sites within a helical surface groove that is lined by conserved residues. The structure reveals the determinants of NLS specificity and suggests a model for the recognition of bipartite NLSs.

==About this Structure==

1BK6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1BK6 OCA].

1BK6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BK6 OCA].

==Reference==

Line 23:

[[Category: protein transport]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 11:43:~~03 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:56:10 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1bk6.gif|left|200px]]<br /><applet load="1bk6" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1bk6.gif|left|200px]]<br /><applet load="1bk6" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1bk6, resolution 2.8&Aring;" />
 '''KARYOPHERIN ALPHA (YEAST) + SV40 T ANTIGEN NLS'''<br />
 ==Overview==
-Selective nuclear import is mediated by nuclear localization signals, (NLSs) and cognate transport factors known as karyopherins or importins., Karyopherin alpha recognizes "classical" monopartite and bipartite NLSs., We report the crystal structure of a 50 kDa fragment of the 60 kDa yeast, karyopherin alpha, in the absence and presence of a monopartite NLS, peptide at 2.2 A and 2.8 A resolution, respectively. The structure shows a, tandem array of ten armadillo repeats, organized in a right-handed, superhelix of helices. Binding of the NLS peptide occurs at two sites, within a helical surface groove that is lined by conserved residues. The, structure reveals the determinants of NLS specificity and suggests a model, for the recognition of bipartite NLSs.
+Selective nuclear import is mediated by nuclear localization signals (NLSs) and cognate transport factors known as karyopherins or importins. Karyopherin alpha recognizes "classical" monopartite and bipartite NLSs. We report the crystal structure of a 50 kDa fragment of the 60 kDa yeast karyopherin alpha, in the absence and presence of a monopartite NLS peptide at 2.2 A and 2.8 A resolution, respectively. The structure shows a tandem array of ten armadillo repeats, organized in a right-handed superhelix of helices. Binding of the NLS peptide occurs at two sites within a helical surface groove that is lined by conserved residues. The structure reveals the determinants of NLS specificity and suggests a model for the recognition of bipartite NLSs.
 ==About this Structure==
-BK6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BK6 OCA].
+BK6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BK6 OCA].
 ==Reference==
@@ Line 23: / Line 23: @@
 [[Category: protein transport]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:43:03 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:56:10 2008''