1bl5: Difference between revisions

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==Overview==

The structure of a rate-limited product complex formed during a single, initial round of turnover by isocitrate dehydrogenase has been determined., Photolytic liberation of either caged substrate or caged cofactor and Laue, X-ray data collection were used to visualize the complex, which has a, minimum half-life of approximately 10 milliseconds. The experiment was, conducted with three different photoreactive compounds, each possessing a, unique mechanism leading to the formation of the enzyme-substrate (ES), complex. Photoreaction efficiency and subsequent substrate affinities and, binding rates in the crystal are critical parameters for these, experiments. The structure suggests that CO2 dissociation is a rapid event, that may help drive product formation, and that small conformational, changes may contribute to slow product release.

The structure of a rate-limited product complex formed during a single initial round of turnover by isocitrate dehydrogenase has been determined. Photolytic liberation of either caged substrate or caged cofactor and Laue X-ray data collection were used to visualize the complex, which has a minimum half-life of approximately 10 milliseconds. The experiment was conducted with three different photoreactive compounds, each possessing a unique mechanism leading to the formation of the enzyme-substrate (ES) complex. Photoreaction efficiency and subsequent substrate affinities and binding rates in the crystal are critical parameters for these experiments. The structure suggests that CO2 dissociation is a rapid event that may help drive product formation, and that small conformational changes may contribute to slow product release.

==About this Structure==

Line 16:

[[Category: Brubaker, M.]]

[[Category: Cohen, B.]]

[[Category: Junior, D.E.Koshland.]]

[[Category: Junior, D E.Koshland.]]

[[Category: Mesecar, A.]]

[[Category: Stoddard, B.L.]]

[[Category: Stoddard, B L.]]

[[Category: AKG]]

[[Category: MG]]

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[[Category: phosphorylation]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:56:27 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The structure of a rate-limited product complex formed during a single, initial round of turnover by isocitrate dehydrogenase has been determined., Photolytic liberation of either caged substrate or caged cofactor and Laue, X-ray data collection were used to visualize the complex, which has a, minimum half-life of approximately 10 milliseconds. The experiment was, conducted with three different photoreactive compounds, each possessing a, unique mechanism leading to the formation of the enzyme-substrate (ES), complex. Photoreaction efficiency and subsequent substrate affinities and, binding rates in the crystal are critical parameters for these, experiments. The structure suggests that CO2 dissociation is a rapid event, that may help drive product formation, and that small conformational, changes may contribute to slow product release.
+The structure of a rate-limited product complex formed during a single initial round of turnover by isocitrate dehydrogenase has been determined. Photolytic liberation of either caged substrate or caged cofactor and Laue X-ray data collection were used to visualize the complex, which has a minimum half-life of approximately 10 milliseconds. The experiment was conducted with three different photoreactive compounds, each possessing a unique mechanism leading to the formation of the enzyme-substrate (ES) complex. Photoreaction efficiency and subsequent substrate affinities and binding rates in the crystal are critical parameters for these experiments. The structure suggests that CO2 dissociation is a rapid event that may help drive product formation, and that small conformational changes may contribute to slow product release.
 ==About this Structure==
@@ Line 16: / Line 16: @@
 [[Category: Brubaker, M.]]
 [[Category: Cohen, B.]]
-[[Category: Junior, D.E.Koshland.]]
+[[Category: Junior, D E.Koshland.]]
 [[Category: Mesecar, A.]]
-[[Category: Stoddard, B.L.]]
+[[Category: Stoddard, B L.]]
 [[Category: AKG]]
 [[Category: MG]]
@@ Line 26: / Line 26: @@
 [[Category: phosphorylation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:33:16 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:56:27 2008''