1bm9: Difference between revisions

Line 1:

[[Image:1bm9.jpg|left|200px]]<br /><applet load="1bm9" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1bm9.jpg|left|200px]]<br /><applet load="1bm9" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1bm9, resolution 2.00Å" />

'''REPLICATION TERMINATOR PROTEIN FROM BACILLUS SUBTILIS'''<br />

==Overview==

The crystal structure of the replication terminator protein (RTP) of B., subtilis has been determined at 2.6 A resolution. As previously suggested, by both biochemical and biophysical studies, the molecule exists as a, symmetric dimer and is in the alpha + beta protein-folding class. The, protein has several uncommon features, including an antiparallel, coiled-coil, which serves as the dimerization domain, and both an, alpha-helix and a beta-ribbon suitably positioned to interact with the, major and minor grooves of B-DNA. A site has been identified on the, surface of RTP that is biochemically and positionally suitable for, interaction with the replication-specific helicase. Other features of the, structure are consistent with the polar contrahelicase mechanism of the, protein. A model of the interaction between RTP and its cognate DNA is, presented.

The crystal structure of the replication terminator protein (RTP) of B. subtilis has been determined at 2.6 A resolution. As previously suggested by both biochemical and biophysical studies, the molecule exists as a symmetric dimer and is in the alpha + beta protein-folding class. The protein has several uncommon features, including an antiparallel coiled-coil, which serves as the dimerization domain, and both an alpha-helix and a beta-ribbon suitably positioned to interact with the major and minor grooves of B-DNA. A site has been identified on the surface of RTP that is biochemically and positionally suitable for interaction with the replication-specific helicase. Other features of the structure are consistent with the polar contrahelicase mechanism of the protein. A model of the interaction between RTP and its cognate DNA is presented.

==About this Structure==

1BM9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1BM9 OCA].

1BM9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BM9 OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Bastia, D.]]

[[Category: Bussiere, D.E.]]

[[Category: Bussiere, D E.]]

[[Category: White, S.]]

[[Category: contrahelicase]]

[[Category: dna-binding protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 11:45:~~19 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:56:46 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1bm9.jpg|left|200px]]<br /><applet load="1bm9" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1bm9.jpg|left|200px]]<br /><applet load="1bm9" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1bm9, resolution 2.00&Aring;" />
 '''REPLICATION TERMINATOR PROTEIN FROM BACILLUS SUBTILIS'''<br />
 ==Overview==
-The crystal structure of the replication terminator protein (RTP) of B., subtilis has been determined at 2.6 A resolution. As previously suggested, by both biochemical and biophysical studies, the molecule exists as a, symmetric dimer and is in the alpha + beta protein-folding class. The, protein has several uncommon features, including an antiparallel, coiled-coil, which serves as the dimerization domain, and both an, alpha-helix and a beta-ribbon suitably positioned to interact with the, major and minor grooves of B-DNA. A site has been identified on the, surface of RTP that is biochemically and positionally suitable for, interaction with the replication-specific helicase. Other features of the, structure are consistent with the polar contrahelicase mechanism of the, protein. A model of the interaction between RTP and its cognate DNA is, presented.
+The crystal structure of the replication terminator protein (RTP) of B. subtilis has been determined at 2.6 A resolution. As previously suggested by both biochemical and biophysical studies, the molecule exists as a symmetric dimer and is in the alpha + beta protein-folding class. The protein has several uncommon features, including an antiparallel coiled-coil, which serves as the dimerization domain, and both an alpha-helix and a beta-ribbon suitably positioned to interact with the major and minor grooves of B-DNA. A site has been identified on the surface of RTP that is biochemically and positionally suitable for interaction with the replication-specific helicase. Other features of the structure are consistent with the polar contrahelicase mechanism of the protein. A model of the interaction between RTP and its cognate DNA is presented.
 ==About this Structure==
-BM9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BM9 OCA].
+BM9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BM9 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Bastia, D.]]
-[[Category: Bussiere, D.E.]]
+[[Category: Bussiere, D E.]]
 [[Category: White, S.]]
 [[Category: contrahelicase]]
 [[Category: dna-binding protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:45:19 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:56:46 2008''