1bvs: Difference between revisions

Line 1:

[[Image:1bvs.gif|left|200px]]<br /><applet load="1bvs" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1bvs.gif|left|200px]]<br /><applet load="1bvs" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1bvs, resolution 3.00Å" />

'''RUVA COMPLEXED TO A HOLLIDAY JUNCTION.'''<br />

==Overview==

Holliday junctions occur as intermediates in homologous recombination and, DNA repair. In bacteria, resolution of Holliday junctions is accomplished, by the RuvABC system, consisting of a junction-specific helicase complex, RuvAB, which promotes branch migration, and a junction-specific, endonuclease RuvC, which nicks two strands. The crystal structure of a, complex between the RuvA protein of M. leprae and a synthetic four-way, junction has now been determined. Rather than binding on the open surface, of a RuvA tetramer as previously suggested, the DNA is sandwiched between, two RuvA tetramers, which form a closed octameric shell, stabilized by a, conserved tetramer-tetramer interface. Interactions between the DNA, backbone and helix-hairpin-helix motifs from both tetramers suggest a, mechanism for strand separation promoted by RuvA.

Holliday junctions occur as intermediates in homologous recombination and DNA repair. In bacteria, resolution of Holliday junctions is accomplished by the RuvABC system, consisting of a junction-specific helicase complex RuvAB, which promotes branch migration, and a junction-specific endonuclease RuvC, which nicks two strands. The crystal structure of a complex between the RuvA protein of M. leprae and a synthetic four-way junction has now been determined. Rather than binding on the open surface of a RuvA tetramer as previously suggested, the DNA is sandwiched between two RuvA tetramers, which form a closed octameric shell, stabilized by a conserved tetramer-tetramer interface. Interactions between the DNA backbone and helix-hairpin-helix motifs from both tetramers suggest a mechanism for strand separation promoted by RuvA.

==About this Structure==

1BVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_leprae Mycobacterium leprae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1BVS OCA].

1BVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_leprae Mycobacterium leprae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BVS OCA].

==Reference==

Line 13:

[[Category: Mycobacterium leprae]]

[[Category: Single protein]]

[[Category: Pearl, L.H.]]

[[Category: Pearl, L H.]]

[[Category: Roe, S.M.]]

[[Category: Roe, S M.]]

[[Category: branch migration]]

[[Category: dna repair]]

[[Category: holliday junction resolvase component]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 11:57:~~33 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:59:37 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1bvs.gif|left|200px]]<br /><applet load="1bvs" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1bvs.gif|left|200px]]<br /><applet load="1bvs" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1bvs, resolution 3.00&Aring;" />
 '''RUVA COMPLEXED TO A HOLLIDAY JUNCTION.'''<br />
 ==Overview==
-Holliday junctions occur as intermediates in homologous recombination and, DNA repair. In bacteria, resolution of Holliday junctions is accomplished, by the RuvABC system, consisting of a junction-specific helicase complex, RuvAB, which promotes branch migration, and a junction-specific, endonuclease RuvC, which nicks two strands. The crystal structure of a, complex between the RuvA protein of M. leprae and a synthetic four-way, junction has now been determined. Rather than binding on the open surface, of a RuvA tetramer as previously suggested, the DNA is sandwiched between, two RuvA tetramers, which form a closed octameric shell, stabilized by a, conserved tetramer-tetramer interface. Interactions between the DNA, backbone and helix-hairpin-helix motifs from both tetramers suggest a, mechanism for strand separation promoted by RuvA.
+Holliday junctions occur as intermediates in homologous recombination and DNA repair. In bacteria, resolution of Holliday junctions is accomplished by the RuvABC system, consisting of a junction-specific helicase complex RuvAB, which promotes branch migration, and a junction-specific endonuclease RuvC, which nicks two strands. The crystal structure of a complex between the RuvA protein of M. leprae and a synthetic four-way junction has now been determined. Rather than binding on the open surface of a RuvA tetramer as previously suggested, the DNA is sandwiched between two RuvA tetramers, which form a closed octameric shell, stabilized by a conserved tetramer-tetramer interface. Interactions between the DNA backbone and helix-hairpin-helix motifs from both tetramers suggest a mechanism for strand separation promoted by RuvA.
 ==About this Structure==
-BVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_leprae Mycobacterium leprae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BVS OCA].
+BVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_leprae Mycobacterium leprae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BVS OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Mycobacterium leprae]]
 [[Category: Single protein]]
-[[Category: Pearl, L.H.]]
+[[Category: Pearl, L H.]]
-[[Category: Roe, S.M.]]
+[[Category: Roe, S M.]]
 [[Category: branch migration]]
 [[Category: dna repair]]
 [[Category: holliday junction resolvase component]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:57:33 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:59:37 2008''