1c11: Difference between revisions
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New page: left|200px<br /><applet load="1c11" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c11" /> '''INTERCALATED D(TCCCGTTTCCA) DIMER, NMR, 7 ST... |
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[[Image:1c11.gif|left|200px]]<br /><applet load="1c11" size=" | [[Image:1c11.gif|left|200px]]<br /><applet load="1c11" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1c11" /> | caption="1c11" /> | ||
'''INTERCALATED D(TCCCGTTTCCA) DIMER, NMR, 7 STRUCTURES'''<br /> | '''INTERCALATED D(TCCCGTTTCCA) DIMER, NMR, 7 STRUCTURES'''<br /> | ||
==Overview== | ==Overview== | ||
The solution structures of the oligodeoxynucleotides d(CCCGTTTCC) and | The solution structures of the oligodeoxynucleotides d(CCCGTTTCC) and d(TCCCGTTTCCA) have been determined by two-dimensional NMR spectroscopy. These oligomers are part of a DNA box in human centromeric alpha satellite targeted by the centromere protein B (CENP-B). Both CENP-B and its recognition box in alphoid DNA are conserved in mammals, suggesting an important biological role. At acidic pH, d(CCCGTTTCC), d(TCCCGTTTCCA) and the full d(TCCCGTTTCCAACGAAG) CENP-B box strand all fold and dimerize in solution forming a stable bimolecular structure containing two GTTT hairpin loops that interact through a novel T : G : G : T tetrad. The stem region of the dimer is a four-stranded intercalated motif in which the hairpin monomers are parallel and held together by C : C+ hydrogen-bonding and intercalation. The loops are at the same end of the dimer and lie across the narrow grooves of the tetraplex. They are remarkably structured and stabilized by base-base cross-stacking, sugar-base stacking, and parallel G:G and antiparallel G:T pairing. In the d(TCCCGTTTCCA)2 structure, the intercalated motif is continued at the other end of the dimer with unpaired but stacked adenine and thymine bases. The possible biological implications of these structures are discussed.Copyright 1997 Academic Press Limited | ||
==About this Structure== | ==About this Structure== | ||
1C11 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | 1C11 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C11 OCA]. | ||
==Reference== | ==Reference== | ||
Centromeric pyrimidine strands fold into an intercalated motif by forming a double hairpin with a novel T:G:G:T tetrad: solution structure of the d(TCCCGTTTCCA) dimer., Gallego J, Chou SH, Reid BR, J Mol Biol. 1997 Nov 7;273(4):840-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9367776 9367776] | Centromeric pyrimidine strands fold into an intercalated motif by forming a double hairpin with a novel T:G:G:T tetrad: solution structure of the d(TCCCGTTTCCA) dimer., Gallego J, Chou SH, Reid BR, J Mol Biol. 1997 Nov 7;273(4):840-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9367776 9367776] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Chou, S | [[Category: Chou, S H.]] | ||
[[Category: Gallego, J.]] | [[Category: Gallego, J.]] | ||
[[Category: Reid, B | [[Category: Reid, B R.]] | ||
[[Category: alphoid dna]] | [[Category: alphoid dna]] | ||
[[Category: centromere]] | [[Category: centromere]] | ||
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[[Category: i-motif]] | [[Category: i-motif]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:01:16 2008'' | ||
