1cd8: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
A secreted fragment of the extracellular portion of human CD8 alpha has | A secreted fragment of the extracellular portion of human CD8 alpha has been expressed in CHO cells, and a deglycosylated and proteolyzed form of this fragment has been crystallized. We report here the crystal structure of this fragment as refined at 2.6 A resolution. The structure was solved by molecular replacement using a superposition of ten variable domains from immunoglobulin light chains as the search model. Only the N-terminal 114 amino acids of CD8 alpha are visible in the electron density maps. The domain formed by these residues possesses a fold typical of immunoglobulin variable domains and associates to form Fv-like homodimers. | ||
==Disease== | ==Disease== | ||
| Line 16: | Line 16: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Axel, R.]] | [[Category: Axel, R.]] | ||
[[Category: Hendrickson, W | [[Category: Hendrickson, W A.]] | ||
[[Category: Leahy, D | [[Category: Leahy, D J.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: surface glycoprotein]] | [[Category: surface glycoprotein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:04:50 2008'' | ||
Revision as of 10:04, 21 February 2008
|
CRYSTAL STRUCTURE OF A SOLUBLE FORM OF THE HUMAN T CELL CO-RECEPTOR CD8 AT 2.6 ANGSTROMS RESOLUTION
Overview
A secreted fragment of the extracellular portion of human CD8 alpha has been expressed in CHO cells, and a deglycosylated and proteolyzed form of this fragment has been crystallized. We report here the crystal structure of this fragment as refined at 2.6 A resolution. The structure was solved by molecular replacement using a superposition of ten variable domains from immunoglobulin light chains as the search model. Only the N-terminal 114 amino acids of CD8 alpha are visible in the electron density maps. The domain formed by these residues possesses a fold typical of immunoglobulin variable domains and associates to form Fv-like homodimers.
Disease
Known diseases associated with this structure: CD8 deficiency, familial OMIM:[186910], Megakaryoblastic leukemia, acute OMIM:[606078]
About this Structure
1CD8 is a Single protein structure of sequence from [1] with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 A resolution., Leahy DJ, Axel R, Hendrickson WA, Cell. 1992 Mar 20;68(6):1145-62. PMID:1547508
Page seeded by OCA on Thu Feb 21 12:04:50 2008
