1cxc: Difference between revisions

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[[Image:1cxc.jpg|left|200px]]<br /><applet load="1cxc" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1cxc.jpg|left|200px]]<br /><applet load="1cxc" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1cxc, resolution 1.6Å" />

'''CRYSTALLIZATION AND X-RAY STRUCTURE DETERMINATION OF CYTOCHROME C2 FROM RHODOBACTER SPHAEROIDES IN THREE CRYSTAL FORMS'''<br />

==Overview==

Cytochrome c(2) serves as the secondary electron donor that reduces the, photo-oxidized bacteriochlorophyll dimer in photosynthetic bacteria., Cytochrome c(2) from Rhodobacter sphaeroides has been crystallized in, three different forms. At high ionic strength, crystals of a hexagonal, space group (P6(1)22) were obtained, while at low ionic strength, triclinic (P1) and tetragonal (P4(1)2(1)2) crystals were formed. The, three-dimensional structures of the cytochrome in all three crystal forms, have been determined by X-ray diffraction at resolutions of 2.20 A, (hexagonal), 1.95 A, (triclinic) and 1.53 A (tetragonal). The most, significant difference observed was the binding of an imidazole molecule, to the iron atom of the heme group in the hexagonal structure. This, binding displaces the sulfur atom of Met l00, which forms the axial ligand, in the triclinic and tetragonal structures.

Cytochrome c(2) serves as the secondary electron donor that reduces the photo-oxidized bacteriochlorophyll dimer in photosynthetic bacteria. Cytochrome c(2) from Rhodobacter sphaeroides has been crystallized in three different forms. At high ionic strength, crystals of a hexagonal space group (P6(1)22) were obtained, while at low ionic strength, triclinic (P1) and tetragonal (P4(1)2(1)2) crystals were formed. The three-dimensional structures of the cytochrome in all three crystal forms have been determined by X-ray diffraction at resolutions of 2.20 A (hexagonal), 1.95 A, (triclinic) and 1.53 A (tetragonal). The most significant difference observed was the binding of an imidazole molecule to the iron atom of the heme group in the hexagonal structure. This binding displaces the sulfur atom of Met l00, which forms the axial ligand in the triclinic and tetragonal structures.

==About this Structure==

1CXC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1CXC OCA].

1CXC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CXC OCA].

==Reference==

Line 13:

[[Category: Rhodobacter sphaeroides]]

[[Category: Single protein]]

[[Category: Allen, J.P.]]

[[Category: Allen, J P.]]

[[Category: Axelrod, H.L.]]

[[Category: Axelrod, H L.]]

[[Category: Chirino, A.J.]]

[[Category: Chirino, A J.]]

[[Category: Day, M.W.]]

[[Category: Day, M W.]]

[[Category: Feher, G.]]

[[Category: Hsu, B.T.]]

[[Category: Hsu, B T.]]

[[Category: Rees, D.C.]]

[[Category: Rees, D C.]]

[[Category: HEM]]

[[Category: electron transport (cytochrome)]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 12:49:~~30 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:10:44 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1cxc.jpg|left|200px]]<br /><applet load="1cxc" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1cxc.jpg|left|200px]]<br /><applet load="1cxc" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1cxc, resolution 1.6&Aring;" />
 '''CRYSTALLIZATION AND X-RAY STRUCTURE DETERMINATION OF CYTOCHROME C2 FROM RHODOBACTER SPHAEROIDES IN THREE CRYSTAL FORMS'''<br />
 ==Overview==
-Cytochrome c(2) serves as the secondary electron donor that reduces the, photo-oxidized bacteriochlorophyll dimer in photosynthetic bacteria., Cytochrome c(2) from Rhodobacter sphaeroides has been crystallized in, three different forms. At high ionic strength, crystals of a hexagonal, space group (P6(1)22) were obtained, while at low ionic strength, triclinic (P1) and tetragonal (P4(1)2(1)2) crystals were formed. The, three-dimensional structures of the cytochrome in all three crystal forms, have been determined by X-ray diffraction at resolutions of 2.20 A, (hexagonal), 1.95 A, (triclinic) and 1.53 A (tetragonal). The most, significant difference observed was the binding of an imidazole molecule, to the iron atom of the heme group in the hexagonal structure. This, binding displaces the sulfur atom of Met l00, which forms the axial ligand, in the triclinic and tetragonal structures.
+Cytochrome c(2) serves as the secondary electron donor that reduces the photo-oxidized bacteriochlorophyll dimer in photosynthetic bacteria. Cytochrome c(2) from Rhodobacter sphaeroides has been crystallized in three different forms. At high ionic strength, crystals of a hexagonal space group (P6(1)22) were obtained, while at low ionic strength, triclinic (P1) and tetragonal (P4(1)2(1)2) crystals were formed. The three-dimensional structures of the cytochrome in all three crystal forms have been determined by X-ray diffraction at resolutions of 2.20 A (hexagonal), 1.95 A, (triclinic) and 1.53 A (tetragonal). The most significant difference observed was the binding of an imidazole molecule to the iron atom of the heme group in the hexagonal structure. This binding displaces the sulfur atom of Met l00, which forms the axial ligand in the triclinic and tetragonal structures.
 ==About this Structure==
-CXC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CXC OCA].
+CXC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CXC OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Rhodobacter sphaeroides]]
 [[Category: Single protein]]
-[[Category: Allen, J.P.]]
+[[Category: Allen, J P.]]
-[[Category: Axelrod, H.L.]]
+[[Category: Axelrod, H L.]]
-[[Category: Chirino, A.J.]]
+[[Category: Chirino, A J.]]
-[[Category: Day, M.W.]]
+[[Category: Day, M W.]]
 [[Category: Feher, G.]]
-[[Category: Hsu, B.T.]]
+[[Category: Hsu, B T.]]
-[[Category: Rees, D.C.]]
+[[Category: Rees, D C.]]
 [[Category: HEM]]
 [[Category: electron transport (cytochrome)]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:49:30 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:10:44 2008''