1d24: Difference between revisions
New page: left|200px<br /><applet load="1d24" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d24, resolution 1.900Å" /> '''CRYSTAL AND MOLECUL... |
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[[Image:1d24.jpg|left|200px]]<br /><applet load="1d24" size=" | [[Image:1d24.jpg|left|200px]]<br /><applet load="1d24" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1d24, resolution 1.900Å" /> | caption="1d24, resolution 1.900Å" /> | ||
'''CRYSTAL AND MOLECULAR STRUCTURE OF A DNA DUPLEX CONTAINING THE CARCINOGENIC LESION O6-METHYLGUANINE'''<br /> | '''CRYSTAL AND MOLECULAR STRUCTURE OF A DNA DUPLEX CONTAINING THE CARCINOGENIC LESION O6-METHYLGUANINE'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal and molecular structure of the first DNA duplex containing the | The crystal and molecular structure of the first DNA duplex containing the carcinogenic lesion O6MeG has been determined to a resolution of 1.9 A and refined to an R factor of 19%. (d[CGC-(O6Me)GCG])2 crystallizes in the left-handed Z DNA form and has crystal parameters and conformational features similar to those of the parent sequence [d(CG)3]2. The methyl groups on O6 of G4 and G10 have C5-C6-O6-O6Me torsion angles of 73 degrees and 56 degrees, respectively, and protrude onto the major groove surface. The base-pairing conformation for the methylated G.C base pairs is of the Watson-Crick type as opposed to a wobble-type conformation that had been proposed in a B DNA fragment. As in other Z DNA structures, a spine of hydration is seen in the minor groove. | ||
==About this Structure== | ==About this Structure== | ||
1D24 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | 1D24 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D24 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal and molecular structure of a DNA duplex containing the carcinogenic lesion O6-methylguanine., Ginell SL, Kuzmich S, Jones RA, Berman HM, Biochemistry. 1990 Nov 20;29(46):10461-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2271657 2271657] | Crystal and molecular structure of a DNA duplex containing the carcinogenic lesion O6-methylguanine., Ginell SL, Kuzmich S, Jones RA, Berman HM, Biochemistry. 1990 Nov 20;29(46):10461-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2271657 2271657] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Berman, H | [[Category: Berman, H M.]] | ||
[[Category: Ginell, S | [[Category: Ginell, S L.]] | ||
[[Category: Jones, R | [[Category: Jones, R A.]] | ||
[[Category: Kuzmich, S.]] | [[Category: Kuzmich, S.]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
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[[Category: z-dna]] | [[Category: z-dna]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:12:11 2008'' | ||
Revision as of 10:12, 21 February 2008
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CRYSTAL AND MOLECULAR STRUCTURE OF A DNA DUPLEX CONTAINING THE CARCINOGENIC LESION O6-METHYLGUANINE
Overview
The crystal and molecular structure of the first DNA duplex containing the carcinogenic lesion O6MeG has been determined to a resolution of 1.9 A and refined to an R factor of 19%. (d[CGC-(O6Me)GCG])2 crystallizes in the left-handed Z DNA form and has crystal parameters and conformational features similar to those of the parent sequence [d(CG)3]2. The methyl groups on O6 of G4 and G10 have C5-C6-O6-O6Me torsion angles of 73 degrees and 56 degrees, respectively, and protrude onto the major groove surface. The base-pairing conformation for the methylated G.C base pairs is of the Watson-Crick type as opposed to a wobble-type conformation that had been proposed in a B DNA fragment. As in other Z DNA structures, a spine of hydration is seen in the minor groove.
About this Structure
1D24 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal and molecular structure of a DNA duplex containing the carcinogenic lesion O6-methylguanine., Ginell SL, Kuzmich S, Jones RA, Berman HM, Biochemistry. 1990 Nov 20;29(46):10461-5. PMID:2271657
Page seeded by OCA on Thu Feb 21 12:12:11 2008
