1d90: Difference between revisions

Line 1:

[[Image:1d90.gif|left|200px]]<br /><applet load="1d90" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1d90.gif|left|200px]]<br /><applet load="1d90" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1d90, resolution 1.700Å" />

'''REFINED CRYSTAL STRUCTURE OF AN OCTANUCLEOTIDE DUPLEX WITH I.T MISMATCHED BASE PAIRS'''<br />

==Overview==

The structure of the synthetic deoxyoctamer d(GGIGCTCC) has been, determined by single crystal X-ray diffraction techniques to a resolution, of 1.7A. The sequence crystallises in space group P6(1), with unit cell, dimensions a = b = 45.07, c = 45.49A. The refinement converged with a, crystallographic residual R = 0.14 and the location of 81 solvent, molecules. The octamer forms an A-DNA duplex with 6 Watson-Crick (G.C), base pairs and 2 inosine-thymine (I.T) pairs. Refinement of the structure, shows it to be essentially isomorphous with that reported for d(GGGGCTCC), with the mispairs adopting a "wobble" conformation. Conformational, parameters and base stacking interactions are compared to those for the, native duplex d(GGGGCCCC) and other similar sequences. A rationale for the, apparent increased crystal packing efficiency and lattice stability of the, I.T octamer is given.

The structure of the synthetic deoxyoctamer d(GGIGCTCC) has been determined by single crystal X-ray diffraction techniques to a resolution of 1.7A. The sequence crystallises in space group P6(1), with unit cell dimensions a = b = 45.07, c = 45.49A. The refinement converged with a crystallographic residual R = 0.14 and the location of 81 solvent molecules. The octamer forms an A-DNA duplex with 6 Watson-Crick (G.C) base pairs and 2 inosine-thymine (I.T) pairs. Refinement of the structure shows it to be essentially isomorphous with that reported for d(GGGGCTCC) with the mispairs adopting a "wobble" conformation. Conformational parameters and base stacking interactions are compared to those for the native duplex d(GGGGCCCC) and other similar sequences. A rationale for the apparent increased crystal packing efficiency and lattice stability of the I.T octamer is given.

==About this Structure==

1D90 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1D90 OCA].

1D90 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D90 OCA].

==Reference==

Line 14:

[[Category: Aymani, J.]]

[[Category: Brown, T.]]

[[Category: Cruse, W.B.T.]]

[[Category: Cruse, W B.T.]]

[[Category: Jack, A.G.C.]]

[[Category: Jack, A G.C.]]

[[Category: Kennard, O.]]

[[Category: Leonard, G.A.]]

[[Category: Leonard, G A.]]

[[Category: a-dna]]

[[Category: double helix]]

Line 23:

[[Category: modified]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 02~~:46:~~40 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:13 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1d90.gif|left|200px]]<br /><applet load="1d90" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1d90.gif|left|200px]]<br /><applet load="1d90" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1d90, resolution 1.700&Aring;" />
 '''REFINED CRYSTAL STRUCTURE OF AN OCTANUCLEOTIDE DUPLEX WITH I.T MISMATCHED BASE PAIRS'''<br />
 ==Overview==
-The structure of the synthetic deoxyoctamer d(GGIGCTCC) has been, determined by single crystal X-ray diffraction techniques to a resolution, of 1.7A. The sequence crystallises in space group P6(1), with unit cell, dimensions a = b = 45.07, c = 45.49A. The refinement converged with a, crystallographic residual R = 0.14 and the location of 81 solvent, molecules. The octamer forms an A-DNA duplex with 6 Watson-Crick (G.C), base pairs and 2 inosine-thymine (I.T) pairs. Refinement of the structure, shows it to be essentially isomorphous with that reported for d(GGGGCTCC), with the mispairs adopting a "wobble" conformation. Conformational, parameters and base stacking interactions are compared to those for the, native duplex d(GGGGCCCC) and other similar sequences. A rationale for the, apparent increased crystal packing efficiency and lattice stability of the, I.T octamer is given.
+The structure of the synthetic deoxyoctamer d(GGIGCTCC) has been determined by single crystal X-ray diffraction techniques to a resolution of 1.7A. The sequence crystallises in space group P6(1), with unit cell dimensions a = b = 45.07, c = 45.49A. The refinement converged with a crystallographic residual R = 0.14 and the location of 81 solvent molecules. The octamer forms an A-DNA duplex with 6 Watson-Crick (G.C) base pairs and 2 inosine-thymine (I.T) pairs. Refinement of the structure shows it to be essentially isomorphous with that reported for d(GGGGCTCC) with the mispairs adopting a "wobble" conformation. Conformational parameters and base stacking interactions are compared to those for the native duplex d(GGGGCCCC) and other similar sequences. A rationale for the apparent increased crystal packing efficiency and lattice stability of the I.T octamer is given.
 ==About this Structure==
-D90 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D90 OCA].
+D90 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D90 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Aymani, J.]]
 [[Category: Brown, T.]]
-[[Category: Cruse, W.B.T.]]
+[[Category: Cruse, W B.T.]]
-[[Category: Jack, A.G.C.]]
+[[Category: Jack, A G.C.]]
 [[Category: Kennard, O.]]
-[[Category: Leonard, G.A.]]
+[[Category: Leonard, G A.]]
 [[Category: a-dna]]
 [[Category: double helix]]
@@ Line 23: / Line 23: @@
 [[Category: modified]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:46:40 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:13 2008''