1ddp: Difference between revisions
New page: left|200px<br /><applet load="1ddp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ddp" /> '''SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [C... |
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'''SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK'''<br /> | '''SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK'''<br /> | ||
==Overview== | ==Overview== | ||
The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin | The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin or cis-DDP) reacts with DNA, cross-linking two purine residues through the N7 atoms, which reside in the major groove in B-form DNA. The solution structure of the short duplex [d(CAT-AGCTATG)]2 cross-linked at the GC:GC site was determined by nuclear magnetic resonance (NMR). The deoxyguanosine-bridging cis-diammineplatinum(II) lies in the minor groove, and the complementary deoxycytidines are extrahelical. The double helix is locally reversed to a left-handed form, and the helix is unwound and bent toward the minor groove. These findings were independently confirmed by results from a phase-sensitive gel electrophoresis bending assay. The NMR structure differs markedly from previously proposed models but accounts for the chemical reactivity, the unwinding, and the bending of cis-DDP interstrand cross-linked DNA and may be important in the formation and repair of these cross-links in chromatin. | ||
==About this Structure== | ==About this Structure== | ||
1DDP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CPT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1DDP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CPT:'>CPT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDP OCA]. | ||
==Reference== | ==Reference== | ||
Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8525382 8525382] | Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8525382 8525382] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Drobny, G | [[Category: Drobny, G P.]] | ||
[[Category: Hopkins, P | [[Category: Hopkins, P B.]] | ||
[[Category: Huang, H.]] | [[Category: Huang, H.]] | ||
[[Category: Reid, B | [[Category: Reid, B R.]] | ||
[[Category: Zhu, L.]] | [[Category: Zhu, L.]] | ||
[[Category: CPT]] | [[Category: CPT]] | ||
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[[Category: nmr]] | [[Category: nmr]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:15:38 2008'' | ||
Revision as of 10:15, 21 February 2008
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SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK
Overview
The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin or cis-DDP) reacts with DNA, cross-linking two purine residues through the N7 atoms, which reside in the major groove in B-form DNA. The solution structure of the short duplex [d(CAT-AGCTATG)]2 cross-linked at the GC:GC site was determined by nuclear magnetic resonance (NMR). The deoxyguanosine-bridging cis-diammineplatinum(II) lies in the minor groove, and the complementary deoxycytidines are extrahelical. The double helix is locally reversed to a left-handed form, and the helix is unwound and bent toward the minor groove. These findings were independently confirmed by results from a phase-sensitive gel electrophoresis bending assay. The NMR structure differs markedly from previously proposed models but accounts for the chemical reactivity, the unwinding, and the bending of cis-DDP interstrand cross-linked DNA and may be important in the formation and repair of these cross-links in chromatin.
About this Structure
1DDP is a Protein complex structure of sequences from [1] with CPT as ligand. Full crystallographic information is available from OCA.
Reference
Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:8525382
Page seeded by OCA on Thu Feb 21 12:15:38 2008
